Solid phase synthesis, cleavage and purification of peptides
Solid phase synthesis, cleavage and purification of peptides
Solid-phase peptide synthesis technology is a key technology of modern protein chemistry, and also a technology of great influence and significance to modern molecular biology and genetic engineering research. The basic process is as follows: 1) Coupling of protected amino group, i.e., the first protected amino group is covalently coupled to the solid phase carrier (which can never be detached during the synthesis process). 2) Deprotection, the protection on the amino group is removed under the condition that the side-bonding group remains stable, exposing the free amino group, e.g., Fmoc is removed. 3) Coupling, the next activated amino acid is condensed with the previous amino acid bonded to solid phase to form a peptide bond. 4) Peptide bond extension, the peptide bond is repeated. Peptide bond lengthening, the two steps of deprotection and coupling are repeated to lengthen the peptide bond to the designed length.5. Cleavage, the synthesized peptide is cut from the solid phase carrier and the side chain protection is removed.6. Purification, the cleaved peptide is purified and identified.
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