Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Operation method
Stained feces by microscopic examination Move I. Protozoan encapsulation staining method For more product details, please visit Aladdin Scientific website.
Drop iodine solution in the center of the slide, pick a small amount of feces with a bamboo skewer, smear it well in the iodine solution, add a coverslip and examine it microscopically.
Cryptosporidium oocyst staining method
Cryptosporidium oocyst staining method can be divided into aureamide-phenol staining method, modified antacid staining method and aureamide-phenol staining-modified antacid staining method.
1. Auramine-phenol staining method
(1) Preparation of staining solution: 1g/L aurantium-phenol staining solution (the first solution): 0.1g of aurantium, 5.0g of carbolic acid, 100ml of distilled water; 3% hydrochloric acid alcohol (the second solution): 3 ml of hydrochloric acid, 100ml of 95% alcohol; 5 g/L potassium permanganate (the third solution): 0.5g of potassium permanganate, 100ml of distilled water.
(2) Staining procedure: add drops of the first solution to the dried fecal membrane, wash after 10-15 minutes; add drops of the second solution, wash after 1 minute; add drops of the third solution, wash after 1 minute and leave to dry; examine under a fluorescence microscope.
2. Modified antacid staining method
(1) Preparation of staining solution: carbonate red staining solution (first solution): 4g of alkaline red, 20ml of 95% alcohol, 8ml of carbonate, 100ml of distilled water; 10% sulfuric acid solution (second solution): 10ml of pure sulfuric acid, 90ml of distilled water (while stirring the sulfuric acid poured into the water); 20 g/L malachite green solution (third solution): 1ml of 20 g/L malachite green solution, 10ml of distilled water. Distilled water 10 ml.
(2) Staining steps: add drops of the first solution to the dried fecal membrane, wash after 1.5-10 minutes; add drops of the second solution, wash after 1-10 minutes; add drops of the third solution, wash after 1 minute and leave to dry; observe under the microscope.