Topic

Coelenterazine

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

3 resources · protocols, FAQs & articles
article

Coelenterazine (CTZ) Differences and an Application Overview

Coelenterazine (Coelenterazine, CTZ, CAS 55779-48-1) is a representative marine luciferin that is widely used as the substrate and light-emitting chromophore for multiple marine luciferases and the jellyfish photoprotein (Aequorin). By tuning spectral position, emission intensity, reaction ...

Updated Mar 202612 min read✓ Reviewed
protocol

BRET (Bioluminescence Resonance Energy Transfer) Method

Biological processes are complex and determined by networks of interactions, called interactome networks, which include protein-protein interactions. The protein interactome is elemental to cellular function, but our full understanding of it is incomplete. One assay that has helped us begin to ...

Updated Aug 202512 min read✓ Reviewed
article

Differences in the Analogues of Coelenterazine

The three primary analogues of coelenterazine—Coelenterazine, Coelenterazine h, and Coelenterazine 400a—can all be employed in similar experimental settings. However, depending on the application, some analogues may yield more optimal results than others.

Updated Aug 202512 min read✓ Reviewed

Related topics

Techniques often explored alongside immunological experiments.

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