Experimental extraction of λ phage DNA from mass cultures using proteinase K and SDS
DNA can be well isolated from large-scale preparations of λ phage by digestion with an effective protease (e.g., proteinase K) and subsequent phenol:chloroform
Topic: DNA experiment
Articles by Topic "DNA experiment"
Knowledge Base
Experimental preparation of λ phage particles from large-scale lysates
λ phage prepared from mass cultures can be recovered from the lysate by polyethylene glycolysis (PEG) precipitation in the presence of high salt. The residual
Gel electrophoresis of formaldehyde psyllids
Formaldehyde-agarose gel electrophoresis (formaldehyde-agarose gel electrophoresis) Formaldehyde is a commonly used denaturant for RNA. It is used for (1) RNA i
denaturing gradient gel electrophoresis
Denaturing gradient gel electrophoresis (DGGE) is a gel system that enables the separation of DNA fragments according to their melting properties. (1) By settin
RAPD (Random Amplified Polymorphic DNA) analysis technique
Polymerase chain reaction (PCR) has greatly influenced almost all areas of molecular biology with its superiority and with its basic procedure and DGGE, a varie
Single Nucleotide Polymorphism (SNP) Assay
SNP (Single Nucleotide Polymorphism), or Single Nucleotide Polymorphism, can be used to detect nucleic acid sequence polymorphisms (Polymorphism) due to changes
In Situ Hybridization Requirements and Procedures
In situ hybridization can be used for (1) solid-phase molecular hybridization and (2) labeled DNA or RNA as probes to detect specific nucleic acid sequences in
Electroshock transformation experiments in Escherichia coli
Electroshock transformation experiments in E. coli can be used for: easier and faster transformation.
Sampling and DNA sequencing
DNA sequences can be enzymatically or chemically degraded to produce a series of sets of DNA fragments that can be resolved by thin-layer denaturing polyacrylam