Dietary restriction, Rapamycin, α-ketoglutarate (AKG), and Carglumic Acid influence aging-related processes through nutrient availability, mTOR signaling, central metabolism, and nitrogen metabolism, respectively. Their effects involve autophagy, proteostasis, mitochondrial function, and ...
Topic
mTOR
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
With the acceleration of population aging, age-related cancers, cardiovascular diseases, neurodegenerative diseases, and metabolic abnormalities continue to increase, making the mechanisms of aging an important direction in life science and disease research. Cellular senescence is an important ...
Aging involves persistent dysregulation of nutrient sensing, energy metabolism, protein homeostasis, and chronic inflammation. IIS, mTOR, AMPK, NF-κB, and Sirtuins are interconnected through nodes including AKT, FOXO, ULK1, and NAD⁺ and jointly regulate cell growth, autophagy, stress ...
Autophagy is a cellular quality control system that delivers cytoplasmic components, damaged organelles, protein aggregates, or intracellular pathogens to lysosomes for degradation. Its core significance is not simply “increased degradation,” but rather the maintenance of cellular ...
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Techniques often explored alongside immunological experiments.