Topic

protein sample

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

2 resources · protocols, FAQs & articles

article

After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?

After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?

Updated Aug 202412 min read✓ Reviewed

article

Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?

Updated Aug 202412 min read✓ Reviewed

After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?

Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?

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