α-Picoline-borane, 2-Methylpyridine borane, PICB
α-Picoline-borane, 2-Methylpyridine borane, PICB
Topic
Reducing agent
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
55 resources · protocols, FAQs & articles
α-Picoline-borane, 2-Methylpyridine borane, PICB
α-Picoline-borane, 2-Methylpyridine borane, PICB
Selenium
Selenium
Red-Al, Sodium bis(2-methoxyethoxy)aluminumhydride
Red-Al,Sodium bis(2-methoxyethoxy)aluminumhydride
Schwartz's Reagent, Zirconocene chloride hydride
Zirconocene chloride hydride is capable of facilitating an efficient reduction process, converting tertiary amides into aldehydes while tolerating the presence of esters. When alkynes and alkenes undergo hydrozirconation with Schwartz's reagent, zirconium-containing products are generated, ...
Rongalite, Sodium hydroxymethanesulfinate
Rongalite generates in situ sulfite anions. Hence, sodium hydroxymethanesulfinate can serve both as a reducing agent and as a reagent for sulfone synthesis.
The Golden Pair for Nucleic Acid Extraction: RNase A and Proteinase K
Nucleic acid extraction is a fundamental process in molecular diagnostics, genetic sequencing, and other related fields. Its core goal is to obtain high-purity, high-integrity nucleic acids. Due to their specificity, RNase A and Proteinase K have become critical auxiliary tools for removing ...
Potassium iodide
Potassium iodide is an ionic compound which is made of the following ions: K+ I−. It crystallises in the sodium chloride structure. It is produced industrially by treating KOH with iodine.
Potassium borohydride
The targeted 1,2-reduction of enones is accomplished by employing sodium borohydride in conjunction with CeCl₃.
Potassium
This reductive coupling process unfolds in two sequential stages. Initially, the coupling reaction is triggered by the transfer of a single electron from an alkali metal (potassium) to the carbonyl groups.
PMHS, Polymethylhydrosiloxane
Polymethylhydrosiloxane (PMHS), a byproduct generated within the silicone manufacturing sector, serves as an economical, user-friendly, and eco-conscious reducing agent. Notably, PMHS exhibits superior stability in the presence of air and moisture compared to alternative silanes, enabling ...
Niobium
Niobium and columbium are interchangeable names for the chemical element possessing the atomic number 41. In 1801, it was named columbium, whereas in 1950, the International Union of Pure and Applied Chemistry officially designated it as niobium (Nb).
Phosphorous acid
Phosphorous acid
Neodymium (low valent)
Neodymium, denoted by the symbol Nd and possessing an atomic number of 60, classifies as a chemical element. As the fourth entity within the lanthanide sequence, it is categorized among the rare-earth metals. Characterized by its hardness and slight malleability, this silvery metal undergoes ...
2-Mercaptoethylamine•HCl (2-MEA)
2-Mercaptoethylamine•HCl (2-MEA) is a mild reducing agent suitable for selectively cleaving hinge-region disulfide bonds of IgG heavy chains. Several popular methods for conjugation or immobilization of antibodies involve covalent attachment specifically through sulfhydryl (−SH) groups.
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP•HCl)
TCEP is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as ...
α-Picoline-borane, 2-Methylpyridine borane, PICB
α-Picoline-borane, 2-Methylpyridine borane, PICB
2,3-dihydroxy-1,4-dithiolbutane (DTT)
Dithiothreitol is the trans isomer of the compound 2,3-dihydroxy-1,4-dithiolbutane. DTT, also known as Cleland’s Reagent, is a cell-permeable, strong but mild reducing agent, with a low redox potential of -0.33 V at pH 7 (optimal pH range 7.1 to 8.0).
Nickel
Nickel
Molybdenum hexacarbonyl
Molybdenum hexacarbonyl
3-Mercaptopropionic acid, 3-MPA
3-Mercaptopropionic acid, 3-MPA
Manganese
A highly effective nickel-catalyzed process enables the direct reductive cross-coupling of equimolar quantities of alkyl and aryl halides in the presence of manganese, demonstrating excellent yield, functional group compatibility, and operational simplicity. This transformation bypasses the ...
Lithium tri-tert-butoxyaluminum hydride, LTBA
Lithium tri-tert-butoxyaluminum hydride, LTBA
Methanol
An anionic iridium complex, [Cp*Ir(2,2'-bpyO)(OH)][Na], serves as a versatile and highly effective catalyst for the transfer hydrogenation of ketones and imines using methanol as the hydrogen source under base-free conditions. The reaction demonstrates excellent tolerance to nitro, cyano, and ...
Magnesium
Magnesium
Lithium
The Birch Reduction provides a means to obtain substituted 1,4-cyclohexadienes.
Lithium triethylborohydride, LiTEBH, Superhydride
LiBHEt3 is an exceptionally potent reducing agent, surpassing even lithium aluminum hydride in efficiency. For instance, LiTEBH facilitates rapid reduction of carbonyl compounds, esters, acid chlorides, acid anhydrides, and tertiary amides into alcohols, as well as the conversion of disulfides ...
Indium (low valent)
Low-valent metal compounds are frequently employed as reducing agents. Given that its most stable oxidation state is 3+, indium is utilized in its 2+ oxidation state to function as a one-electron reducing agent.
LAH, Lithium aluminum hydride, Lithium tetrahydridoaluminate
LAH, Lithium aluminum hydride, Lithium tetrahydridoaluminate
Formaldehyde
Formaldehyde can undergo oxidation or reduction processes with relative ease.
Ethanol
Ethanol
Iron (low valent)
Iron (low valent)
Dimethoxymethylsilane, DMMS
When you use a special type of copper hydride as a catalyst to reduce a certain kind of acid called α,β-unsaturated carboxylic acids, it can produce different kinds of saturated β-chiral aldehydes in good amounts.
Do I need to add extra mercaptoethanol before using this product?
Do I need to add extra mercaptoethanol before using this product?
Diphenylsilane
Diphenylsilane
Does RIPA lysis buffer contain reducing agents such as DTT or mercaptoethanol?
Does RIPA lysis buffer contain reducing agents such as DTT or mercaptoethanol?
Diethylsilane
Diethylsilane
DIBAL-H, Diisobutylaluminium hydride
DIBAL-H, Diisobutylaluminium hydride
1,3-Dimethylimidazol-2-ylidene borane (diMe-Imd-BH3)
NHC-trihydroboranes are white solids that are stable to air, water, and even chromatography. However, 1,3-dimethylimidazol-2-ylidene borane is a surprisingly good hydride donor that is comparable to anionic reagents such as NaCNBH3. The reagent can be used for reductive amination procedures and ...
1, 4-Dioxane
1, 4-dioxane, a heterocyclic organic ether, is a colorless liquid that emits a subtle, sweet aroma reminiscent of diethyl ether. Commonly referred to simply as dioxane due to the scarcity of its other isomers (1, 2- and 1, 3-), this compound finds widespread use as a solvent across numerous ...
Trimethylamine borane / Borane trimethylamine complex
Trimethylamine borane, also known as trimethylamine complex with borane or trimethylamine borane adduct, is a chemical compound with the formula (CH3)3N·BH3. It is a coordination complex formed by the reaction between trimethylamine (N(CH3)3) and borane (BH3).
Bis(neopentylglycolato)diboron, B2nep2
Bis(neopentylglycolato)diboron, B2nep2
Boranes (Dimethylsulfide Borane, Borane-Tetrahydrofuran Complex)
Diborane (B2H6), the simplest borane, is a useful reagent with many applications, but it is pyrophoric, gaseous and not convenient to handle. There is a wide range of boranes: Decaborane for example is not as reactive as diborane and is used as reducing agent too.
Bis(pinacolato)diboron, B2pin2
Bis(pinacolato)diboron is a covalent compound containing two boron atoms and two pinacolato ligands. It has the formula [(CH3)4C2O2B]2; the pinacol groups are sometimes abbreviated as "pin", so the structure is sometimes represented as B2pin2. It is a colourless solid that is soluble in organic ...
1,4-Cyclohexadiene, CHD
1,4-Cyclohexadiene can act as hydrogen atom donor due to aromatization as driving force.
Diethoxymethylsilane, DEMS
Chiral rhodium-bis(oxazolinyl)phenyl complexes catalyze the conjugate hydrosilylation of 3,3-diarylacrylate derivatives to prepare optically active 3,3-diarylpropanoate derivatives in high yields and high enantioselectivities.
Catecholborane
Catecholborane (abbreviated HBcat) is an organoboron compound that is useful in organic synthesis. This colourless liquid is a derivative of catechol and a borane, having the formula C6H4O2BH.
9-Borabicyclo[3.3.1]nonane / 9-BBN
9-Borabicyclo[3.3.1]nonane or 9-BBN is an organoborane compound. This colourless solid is used in organic chemistry as a hydroboration reagent. The compound exists as a hydride-bridged dimer, which easily cleaves in the presence of reducible substrates. 9-BBN is also known by its nickname ...
Aladdin® Reducing Agents
A reducing agent, also known as a reductant, is a substance that has the ability to donate electrons to another substance.
Tetrahydroxydiboron/Bis-Boric Acid/BBA/B2(OH)4
Tetrahydroxydiboron is a reducing agent, that for example slowly produces hydrogen gas in water.
Benzaldehyde
Benzaldehyde (C6H5CHO) is an organic compound consisting of a benzene ring with a formyl substituent. It is among the simplest aromatic aldehydes and one of the most industrially useful.
Thioacetic acid (AcSH)
Thioacetic acid can be used as a mild reducing agent for the deoxygenation of sulfoxides, reduction of azides and azobenzenes.
Alcohol dehydrogenase (ADH)
Dehydrogenases function as enzymes responsible for oxidizing and reducing carbonyl groups, particularly alcohols. These enzymes primarily rely on NAD(P)H for their activity. In the reduction process of aldehydes and ketones, baker yeast is frequently employed.
Ascorbic Acid; Vitamin C
Ascorbic Acid; Vitamin C
Benzyl Alcohol
Benzyl alcohol (also known as α-cresol) is an aromatic alcohol with the formula C6H5CH2OH. The benzyl group is often abbreviated "Bn" (not to be confused with "Bz" which is used for benzoyl); thus benzyl alcohol is denoted as BnOH. Benzyl alcohol is a colorless liquid with a mild pleasant ...
Related topics
Techniques often explored alongside immunological experiments.