Topic

X-Gal

Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.

4 resources · protocols, FAQs & articles
article

β-Galactosidase (β-galactosidase, EC 3.2.1.23) is a glycoside hydrolase family that catalyzes hydrolysis of β-D-galactosidic bonds. It can cleave lactose and related substrates into monosaccharides and, under certain conditions, carry out transgalactosylation to generate products such as ...

Updated Aug 2026·42 min read
article

β-Galactosidase (β-galactosidase, EC 3.2.1.23) is a glycoside hydrolase family that catalyzes hydrolysis of β-D-galactosidic bonds. It can cleave lactose and related substrates into monosaccharides and, under certain conditions, carry out transgalactosylation to generate products such as ...

Updated Jul 2026·9 min read
article

β-Galactosidase (β-galactosidase, EC 3.2.1.23) is a glycoside hydrolase family that catalyzes hydrolysis of β-D-galactosidic bonds. It can cleave lactose and related substrates into monosaccharides and, under certain conditions, carry out transgalactosylation to generate products such as ...

Updated Jan 2026·21 min read
protocol

Blue-white screening of bacterial colonies is a popular and effective molecular biology tool often used to detect recombinant bacteria in cloning experiments. Central to this technique is the enzymatic activity of β-galactosidase, a tetrameric enzyme encoded by the lacZ α gene in E. coli that ...

Updated Aug 2025·21 min read
Related topics

Techniques often explored alongside immunological experiments.

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