ZFR2 Mouse mAb: Target, Applications & Research Use
ZFR2 Mouse mAb: Target, Applications & Research Use
ZFR2 Mouse mAb is a mouse-derived monoclonal antibody targeting the ZFR2 protein, supplied in PBS buffer and designed to deliver reliable, highly specific detection of ZFR2 in a variety of research applications.
- Product Name
- ZFR2 Mouse mAb
- Catalog Number
- Ab351456
- Host Species
- Mouse
- Antibody Type
- Monoclonal Antibody (mAb)
- Formulation
- PBS (Phosphate-Buffered Saline)
- Reference Code
- KC-3816
What Is ZFR2 and Why Does It Matter?
ZFR2 (Zinc Finger RNA-Binding Protein 2) is a member of the zinc finger protein family characterized by conserved zinc-coordinating domains that mediate RNA or DNA binding. Members of this protein family play roles in gene regulation, RNA processing, and cellular signaling pathways. Understanding ZFR2 expression and localization is relevant to research into gene expression regulation, RNA biology, and potentially disease-associated pathways.
Monoclonal antibodies targeting ZFR2, such as the ZFR2 Mouse mAb (Ab351456), enable researchers to detect, quantify, and localize ZFR2 protein in experimental systems, providing a critical tool for dissecting its biological functions.
Product Specifications and Formulation
The ZFR2 Mouse mAb (Ab351456) is a mouse-derived monoclonal antibody formulated in Phosphate-Buffered Saline (PBS). The PBS-only formulation makes it compatible with a wide range of downstream applications without interference from carrier proteins or preservatives that could affect sensitive assays.
Key specifications include:
- Host: Mouse
- Antibody Format: Monoclonal (mAb)
- Formulation: PBS only
- Reference Code: KC-3816
- SKU: Ab351456
Researchers should store the antibody according to the datasheet recommendations to maintain optimal activity and specificity. Aliquoting is advised to avoid repeated freeze-thaw cycles.
Recommended Applications and Usage Guidelines
Monoclonal antibodies formulated in PBS are broadly suitable for standard immunological techniques. Typical applications for a mouse monoclonal antibody of this class may include:
- Western Blotting (WB): Detection of ZFR2 protein in cell or tissue lysates under denaturing conditions.
- Immunofluorescence (IF): Visualization of ZFR2 subcellular localization in fixed cells or tissue sections.
- Immunohistochemistry (IHC): Detection of ZFR2 in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections.
- Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative measurement of ZFR2 in solution-based formats.
- Immunoprecipitation (IP): Pull-down of ZFR2 protein complexes for interaction studies.
Optimal working dilutions should be determined empirically for each application and sample type. Please refer to the product datasheet (KC-3816) for validated conditions.
Tips for Optimal Antibody Performance
To achieve the best results with ZFR2 Mouse mAb, consider the following best practices:
- Storage: Store at -20 °C for long-term preservation. Avoid repeated freeze-thaw cycles by preparing single-use aliquots.
- Blocking: Use an appropriate blocking agent (e.g., 5% non-fat milk or BSA in TBST) to minimize non-specific background signal.
- Secondary Antibody: Pair with a validated anti-mouse IgG secondary antibody conjugated to your detection system of choice (HRP, fluorophore, etc.).
- Positive Controls: Include a cell line or tissue known to express ZFR2 as a positive control in each experiment.
- Titration: Perform a titration experiment to establish the optimal antibody concentration for each application to balance signal and background.
If non-specific bands or high background are observed, consider increasing wash stringency or adjusting antibody concentration accordingly.
Frequently asked questions
What is the formulation of ZFR2 Mouse mAb (Ab351456)?
ZFR2 Mouse mAb is formulated in PBS (Phosphate-Buffered Saline) only, with no additional carrier proteins or preservatives. This clean formulation makes it directly compatible with a broad range of immunological applications.
Which secondary antibody should I use with ZFR2 Mouse mAb?
Since ZFR2 Mouse mAb is produced in mouse, you should use an anti-mouse IgG secondary antibody. Choose a secondary conjugated to your preferred detection label (e.g., HRP for Western blotting, or a fluorophore for immunofluorescence). Ensure the secondary antibody is validated for use with the species of your primary antibody host.
How should ZFR2 Mouse mAb be stored to maintain stability?
The antibody should be stored at -20 °C for long-term stability. To avoid activity loss from repeated freeze-thaw cycles, aliquot the antibody into single-use volumes upon receipt. Avoid prolonged exposure to temperatures above 4 °C.
What applications is ZFR2 Mouse mAb suitable for?
As a monoclonal antibody formulated in PBS, ZFR2 Mouse mAb is potentially suitable for applications including Western blotting, immunofluorescence, immunohistochemistry, ELISA, and immunoprecipitation. Please consult the product datasheet (KC-3816) for validated applications and recommended dilutions.
Where can I find validated protocols for using Ab351456?
Validated protocols and application-specific recommendations for ZFR2 Mouse mAb (Ab351456) are provided in the product datasheet available on the Aladdin Scientific website. You may also contact Aladdin Scientific technical support for personalized guidance.
References
- Aladdin Scientific. ZFR2 Mouse mAb, Cat. No. Ab351456, Reference KC-3816. Product Datasheet.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.