Porcine Embryo Transfer Technology

Summary

Embryo transfer in pigs refers to the technique of removing the early embryo from one sow and transferring it into the reproductive tract of another sow with the same physiological state, so that it develops into a new individual, also known as "artificial insemination" or "borrowing the womb to carry a baby". The individual who provides the embryo is called the donor and the individual who receives the embryo is called the recipient. Embryo transfer is the basis of embryo engineering, which enables us to carry out rapid expansion of good breeding stock, and is also a necessary technical link for the production of transgenic pigs and cloned pigs.

Principle

The principle of embryo transfer is based on several physiological foundations:

① The physiological changes in the reproductive organs of the donor and recipient of the same species of animal are the same, providing the same physiological environment for the transfer of the donor embryo into the recipient;

② Early embryos are free for a certain period of time, providing the possibility of embryo collection;

③ The recipient is basically free from immune rejection of foreign embryos transferred into the uterus, providing the possibility of embryo survival in the recipient;

(iv) The donor embryo can establish normal physiological and organizational links with the recipient uterus, but the genetic characteristics of the donor embryo are not affected in any way during conception.

Operation method

Porcine Embryo Transfer Technology

Principle

The principle of embryo transfer is based on the following physiological bases: (1) the physiological changes in the reproductive organs of the donor and the recipient of the same species of animal are the same, providing the same physiological environment for the transfer of the donor embryo into the recipient; (2) the early embryo is in a free state for a certain period of time, which provides the possibility of embryo collection; (3) basically, the recipient does not have an immune rejection reaction to the foreign embryo transferred into the uterus, which provides a possibility of the embryo's survival in the recipient; (4) the donor embryo can establish normal physiological and tissue connections with the recipient's uterus, but the genetic characteristics of the donor embryo are not affected in any way during gestation. (iv) The donor embryo can establish normal physiological and organizational links with the recipient uterus, but the genetic characteristics of the donor embryo are not affected in any way during gestation.

Materials and Instruments

Equipment:
① Thermal chamber
② Microscope, hot plate
③ 4-well plate and small sterile petri dish
③ 4-well plate and small sterile petri dish ④ Pipette gun, gun tip, sterile blade, scissors
⑤ 1 ml disposable syringe, tin foil, toilet paper, sterilized latex gloves
Reagents:
① Material: pig
② 70% ethanol
② 70% ethanol ③ Operating fluid

Move

The basic process of porcine embryo transfer can be divided into the following steps:
1. The recipient pigs were treated with simultaneous oestrus and pseudo-pregnancy, and each recipient pig was injected with 15 μg of GnRH hormone intramuscularly (oestrus was 22 hours later than the embryo donor), and then was caged together with the ligated boar.
2. Embryos of all periods were transferred to the oviducts, and the trumpet was found, and then the embryos to be transferred were drawn up by a special pipette with an inner diameter of 0.5~1 mm. With 0.05 ml of egg transfer solution carefully inserted into the trumpet tube, slowly put into the oviduct, and then transfer the embryos.
3. Perform oviduct transfer on both sides, and transfer 3-5 embryos on each side.
4. After embryo transfer, the recipient pig should be careful to inject antibiotics twice a day in the first few days, to prevent infection of the wound. At the same time, it should be kept in a single pen to prevent the pigs from fighting with each other and breaking the wound or abortion. The nutrition level of the recipient sows can be matched to the diet of pregnant sows. In the middle and late stages of pregnancy, pay attention to the supplementation of green fodder and keep good observation.
5. Check the pregnancy by abdominal palpation after transplantation.

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