Fecal Test Sedimentation Method

Summary

Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website

Operation method

Fecal Test Sedimentation Method

Principle

Suitable for the detection of most helminth eggs and some protozoan encystments. Can be categorized as natural sedimentation, centrifugal sedimentation and mercury-iodoraldehyde centrifugal sedimentation.

Move

1. Natural sedimentation

(1) Pick 20-30g of feces with a glass rod and filter it through a 40-60 mesh copper sieve into a conical glass measuring cup full of water and let it stand for 25 minutes;

(2) Pour off the upper layer of fecal liquid, leaving only the sediment, and then filled with water, according to every 20 minutes to change the water once, repeated 3 to 4 times, until the upper layer of liquid clarification;

(3) Finally, pour off the upper liquid and take the precipitate on a slide for microscopic examination. If checking the capsule, the water change interval should be extended to 6 hours to change once

2. Centrifugal precipitation method

(1) Place the precipitate in a centrifuge tube after filtering out the crude residue, and centrifuge for 1~2 minutes at 1500~2000 rpm;

(2) Pour off the upper liquid, then add water and sediment mixing, so repeated centrifugation 3 ~ 4 times, until the upper liquid is clear; (3) Pour off the upper liquid, take the precipitate on a slide microscopic inspection

3. Mercury iodine aldehyde centrifugal precipitation method

(1) Take 1g of feces, add appropriate amount (about 10 ml) of mercury iodine aldehyde solution, mix well;

(2) Filter through 2 layers of skimmed gauze, then add 4 ml of ether and shake well for 2 minutes;

(3) Centrifuge (2000 rpm) for 1-2 minutes, then divide into 4 layers: ether, fecal sludge, mercury-iodoraldehyde and sediment;

(4) Aspirate and discard the upper 3 layers, and take the sediment for microscopic examination.

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