What is the purpose of an isotype control? How are they selected?
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What is the purpose of an isotype control? How are they selected?
·Reviewed·Published ·Updated ·8 min read
What is the purpose of an isotype control? How are they selected?
Isoform control antibody is used to eliminate background staining due to non-specific binding of antibody to cell surface Fc receptor, etc. It can be used as a basis for judging negative and positive cells, especially for indicators of low or continuous expression, which are not good for data analysis, and isoform control antibody is an indispensable control. Isotype control is the same as the stained monoclonal antibody (1) the same species source (2) the same immunoglobulin and subtype (3) the same fluorescein labeling (4) the same dose and concentration (5) purified from the serum of unimmunized animals.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "What is the purpose of an isotype control? How are they selected?" Aladdin Knowledge Base, updated Sep 20, 2024. https://www.aladdinsci.com/us_en/faqs/what-is-the-purpose-of-an-isotype-control-en.html
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