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Denhardt Solution (50x) Recipe

Laboratory Protocols & SOPs Library

Denhardt Solution (50x) Recipe

· Published Jul 27, 2023 · Updated Jun 9, 2025

Recipe

1.Add the following to 900ml distilled H2O

10g Ficoll 400

10g Polyvinilpyrolidone

10g BSA

2.Filter solution prior to storage through a 0.2uM filter

3.Store at 4℃

4.Warm to appropriate temperature prior to use

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Denhardt Solution (50x) Recipe" Aladdin Knowledge Base, updated Jun 9, 2025. https://www.aladdinsci.com/us_en/faqs/denhardt-solution-50x-recipe-en.html

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