Membran Substrate - Dark blue precipitates (HRP)

Cat. No.: M491331

GRADE & PURITY Dark blue precipitates (HRP)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Overview

1、Product attribute

Shelf life: 36 months

Marker enzyme: horseradish peroxidase (HRP)

Reaction time：short (up to 20 minutes) at 20-25°C

Lot-to-lot variation：not visible by naked eye

Boiling point : 100℃

pH-Value (at 20 °C):  4.9-5.3

Density (20℃) : 1.013 g/cm³

Water solubility: easily soluble

Appearance: colourless to light yellow liquid

Odour: odourless

Light sensitive
Heat sensitive

2、Requirements for storage rooms and vessels

1.Keep container tightly closed.

2.Keep cool. protected from light

3.Keep/Store only in original container.

4.Never return spills in original containers for reuse.

5. Keep away from: Food and feeding stuffs

6. It is possible to transport the solution at ambient temperature, unless it does not exceed 30 °C. The transport should take less than one week.

7. Contaminated or leaked out substrate solution from damaged bottles should not be used anymore and has to be destroyed.

8.The solution still works even beyond the expiry date, but a lower activity has to be taken in account.

3、Effective Components and Principle of Function

A citrate buffer system (pH 5.0) contains the following effective components

· Hydrogen peroxide (<3.0 mM)

· 3,3`,5,5`-Tetramethylbenzidine (TMB, 1.2 mM)

· precipitating reagent ( 5 g/l)

Peroxidase catalyses the decomposition of hydrogen peroxide, taking electrons from TMB (oxidation). By oxidation TMB forms a radical cation that stabilizes by dimerisation and shows the typical blue colour.

These blue cation is stabilized by forming a insoluble complex with the precipitating reagent. So at positions with activity of horseradish peroxidase a blue to violet precipitate will appear.

4、Biosafety information

This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；

The substances in the mixture do not meet the PBT/vPvB criteriaaccording to REACH, annex XIII.

5、Advantage

1. Rapid precipitate formation due to high activity

2. Very low background contributes to a high contrast

3. Dark blue precipitates

4. No significant fading after reaction stop

5. MembraneReuse of membranes after stripping for further immune

6、Instruction for usage

For bottling consider the following instructions:

• Work in a dust free and darkened room.

• Pay attention that the solution has no contact with metal parts (leading to catalysis) of your instruments. Closed systems of silicon tubes are favoured.

• Clean all instruments and vessels very carefully.

• Never touch parts of the instruments that are in contact with the solution with the naked hand. Wear powder free gloves.

• Close the bottles immediately to minimize the influence of light and dust.

• Use bottles that are not permeable to light, made from HDPE or PP.

7、 General Instructions for the Use in Blotting Systems 

Only qualified laboratory staff, who are familiar with the basics of immunological methods, are allowed to use this solution.

The substrate solution is intended for the use in blotting systems with visual interpretation. Under defined conditions a densitometric measurement is possible.

Depending from the system it is sufficient to wash the test strips after incubation with conjugate prior to transfer into the substrate solution. The strips are developed by movement on an orbital shaker until a distinct colour appears.

The most commercial membranes allow to be washed and dried and the dried strips may be stored for documentary purposes. (Pay attention: some glues destroy the coloured precipitate)

Specifications

Specifications & Purity

Dark blue precipitates (HRP)

Storage

Store at 2-8°C, Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• PH: 4.9-5.3

Chemical and Physical Properties

• Sensitivity: Light & heat sensitive