Plasminogen from human plasma, CAS No.9001-91-6

CAS: 9001-91-6 Cat. No.: np001027

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), ≥120 U/mg protein

Accession #

P00747

Bioactivity

≥120 U/mg protein. One unit is defined as the amount of the enzyme which hydrolyzes one umole of N-tosyl-L arginine-methyl ester in 30 minutes at 37℃, pH 8.0.

Overview

Found at 12 mg per 100 ml normal plasma. It is the precursor of the serine protease plasmin which s involved in fibrinolysis.

Extinction Coefficient: 1.71

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

The activity of this product is evaluated using a TAME (N-tosyl-L-arginine methyl ester) esterolytic activity assay. Briefly, plasminogen is activated with streptokinase and incubated in 0.1 M Tris buffer (pH 8.0) with the TAME substrate. The reaction mixture is incubated at 37 °C for 15 minutes, and absorbance is measured at 510 nm using a spectrophotometer. The resulting values are plotted linearly to determine the specific activity (U/mg).

One unit of activity is defined as the amount of enzyme that hydrolyzes 1 µmole of TAME substrate in 30 minutes at 37 °C, pH 8.0.

Specifications

Product Name

Plasminogen from human plasma, CAS No.9001-91-6

Synonyms

DKFZp779M0222 | EC 3.4.21 | EC 3.4.21.7 | plasmin | Plasminogen | Plg | HAE4

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), ≥120 U/mg protein

Biochemical and Physiological Mechanisms

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It

Bioactivity

≥120 U/mg protein. One unit is defined as the amount of the enzyme which hydrolyzes one umole of N-tosyl-L arginine-methyl ester in 30 minutes at 37℃, pH 8.0.

Accession #

P00747

Predicted molecular weight

90~92 kDa

Action Type

INHIBITOR

CAS

9001-91-6

Enzyme Commission Number

EC 3.4.21.7

Molecule Type

Enzyme

Storage and Shipping

Shape

Lyophilized from 20 mM Tris-HCl, pH 7.5, with 2 mM EDTA

Concentration

≥120 U/mg protein

Reconstitution

After centrifugation, we suggest addition of de-ionized H2O to the original volume, followed by gentle mixingto ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (5 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

≥ 120 units per mg protein. One unit is defined as the amount of the enzyme which hydrolyzes one umole of N-tosyl-L arginine-methyl ester in 30 minutes at 37oC, pH 8.0.

Images

SDS-PAGE: Plasminogen from human plasma (np001027)

Plasminogen from human plasma (np001027) - SDS-PAGE
3 μg/lane of Plasminogen from human plasma (np001027) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 83 kDa under reducing condition (95℃ Heated) and 72 & 77 kDa under non-reducing condition (95℃ Heated); showing the band at 95 kDa under reducing condition (Not Heated) and 72 & 77 kDa under non-reducing condition (Not Heated).