Pyruvate Kinase (PK), CAS No.9001-59-6

CAS: 9001-59-6 Cat. No.: P774071

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥50 U/mg enzyme powder; ≥300 U/mg protein

Accession #

P11974

Bioactivity

≥50 U/mg enzyme powder; ≥300 U/mg protein

Overview

Pyruvate kinase (PK, EC 2.7.1.40) is a key enzyme in the glycolytic pathway, and its standard catalytic reaction is as follows: Phosphoenolpyruvate (PEP) + ADP → Pyruvate + ATP. 

Properties

Source: Microorganism
Isoelectric point: 5.2
Michaelis constant: 1.1×10^-3 M (ADP); 2.2×10^-3 M (PEP)
Optimum pH: 7.5                     Fig. 1
Optimum temperature: 65℃                      Fig. 3
pH Stability: 5.0~10.0 (37℃, 20hr)                      Fig. 2
Thermal stability: < 60℃ (pH 8.5, 20min)                      Fig. 4
Inhibitors: Ca2+, Co2+,Cu 2+, Fe3+, Mn2+, Ni2+, NEM, Proclin, SDS
Effect of various chemicals:                     Table 1
Reaction:

  

Specifications

Product Name

Pyruvate Kinase (PK), CAS No.9001-59-6

Synonyms

pyruvate kinase | phosphoenolpyruvate kinase | phosphoenol transphosphorylase | PKM | PKM2

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥50 U/mg enzyme powder; ≥300 U/mg protein

Bioactivity

≥50 U/mg enzyme powder; ≥300 U/mg protein

Accession #

P11974

Predicted molecular weight

68 kDa (SDS-PAGE)

CAS

9001-59-6

Enzyme Commission Number

EC 2.7.1.40

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥50 U/mg enzyme powder; ≥300 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for 5 years. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will convert one micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30℃.