Pyruvic Acid (PA) Content Assay Kit (LDH, Colorimetric Method)

Cat. No.: P1515904

GRADE & PURITY BioReagent

Overview

Pyruvic Acid (PA), also known as 2-oxopropanoic acid, is a crucial intermediate in fundamental metabolism across living organisms. It serves as a central metabolic hub, enabling the interconversion of carbohydrates, fats, and amino acids via acetyl-CoA and the tricarboxylic acid (TCA) cycle. Both pyruvate and lactate are end products of anaerobic glycolysis. Researchers often analyze them together, using their ratio to estimate the degree of circulatory failure. Methods for pyruvate detection include the lactate dehydrogenase (LDH) catalytic assay and the dinitrophenylhydrazine method. The latter is an older technique that relies on color formation for easy observation, but it suffers from interference by α-keto acids, poor specificity, and cumbersome procedures. Currently, the lactate dehydrogenase catalytic assay is the preferred method. The detection principle of this kit is based on the LDH-catalyzed oxidation of pyruvate to lactate in the presence of NADH, which generates NAD⁺. Under weakly alkaline conditions, the reaction equilibrium favors the oxidation of pyruvate to lactate. By measuring the rate of decrease in NADH absorbance at 340 nm using a microplate reader, the pyruvate concentration can be calculated. This kit is suitable for detecting endogenous pyruvate levels in whole blood plasma samples. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.

Storage and Shipping

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for 6 months. Store in the dark.