GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ATP5A Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Specifications
Product Name
Recombinant ATP5A Antibody - Primary antibody, specific to ATP5F1A, Rabbit IgG
Synonyms
ATP synthase alpha chain antibody | ATP synthase alpha chain, mitochondrial antibody | ATP synthase subunit alpha antibody | ATP synthase subunit alpha mitochondrial antibody | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, card
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of
Host species
Rabbit
Specificity
ATP5F1A
Immunogen
A synthetic peptide derived from human ATP5A (AA 200-250)
Positive Control
WB: Mouse liver, Rat liver, Rat brain lysates. IF/ICC: HeLa, MCF7 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ATP5A Antibody (Ab090115) - Western Blot All lanes: Recombinant ATP5A Antibody (Ab090115) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa Observed band size: 53 kDa Exposure time: 55.2 s
Recombinant ATP5A Antibody (Ab090115) - Western Blot All lanes: Recombinant ATP5A Antibody (Ab090115) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa Observed band size: 53 kDa Exposure time: 55.2 s
Recombinant ATP5A Antibody (Ab090115) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant ATP5A Antibody (Ab090115) at 1/50 dilution.
Recombinant ATP5A Antibody (Ab090115) - IF/ICC Immunofluorescent analysis of MCF7 cells, using Recombinant ATP5A Antibody (Ab090115) at 1/50 dilution.
Application
Application
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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