GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.6 mg/mL
A synthetic peptide derived from human ATP5G1/G2/G3 (AA 98-110)
Positive Control
WB: Jurkat, HeLa, HEK293, Hep G2, RAW 264.7 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.6 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ATP5G1/G2/G3 Antibody (Ab090129) - Western Blot All lanes: Recombinant ATP5G1/G2/G3 Antibody (Ab090129) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 11 kDa Exposure time: 8.9 s
Recombinant ATP5G1/G2/G3 Antibody (Ab090129) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATP5G1/G2/G3 Antibody (Ab090129). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant ATP5G1/G2/G3 Antibody (Ab090129) - IF/ICC IF analysis of Recombinant ATP5G1/G2/G3 Antibody (Ab090129) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant ATP5G1/G2/G3 Antibody (Ab090129) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/200
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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