GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Dnmt1 Antibody, Recombinant, could be used for WB, IHC and so on.
Specifications
Product Name
Recombinant Dnmt1 Antibody - Primary antibody, specific to DNMT1, Rabbit IgG
Synonyms
AIM | CXXC9 | CXXC9CXXC finger protein 9 | CXXC-type zinc finger protein 9 | DNA (cytosine-5-)-methyltransferase 1 | DNA (cytosine-5)-methyltransferase 1 | DNA methyltransferase 1 | DNA methyltransferase HsaI | DNA MTase HsaI | DNMT | EC 2.1.1.37 | DNMT1
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chro
Host species
Rabbit
Specificity
DNMT1
Immunogen
A synthetic peptide derived from human DNMT1 (AA 1500-1616)
Positive Control
WB: HeLa and Jurkat cell lysates. IHC: Rat spleen, Human lung large cell cancer, Human glioblastoma, Mouse lung tissues.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Dnmt1 Antibody (Ab100220) - Western Blot All lanes: Recombinant Dnmt1 Antibody (Ab100220) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 183 kDa Observed band size: 182, 360 kDa The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19173286). Exposure time: 56.4 s
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Rat spleen, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Mouse lung, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Application
Application
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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