GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
Q5ZPR3-2
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
1、Immobilized Recombinant Human CD276 Protein (rp143912) at 0.5 μg/mL can bind Anti Human CD276 Antibody with the EC50 of 10.73 ng/mL. 2、Immobilized Recombinant Human CD276 Protein (rp143912) at 1 μg/mL can bind Anti Human CD276 Antibody with a linear range of 1 - 4 ng/mL (EC50:4.3 ng/mL).
Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,His-Tag,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Purity >95% SDS-PAGE
Function May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
1、Immobilized Recombinant Human CD276 Protein (rp143912) at 0.5 μg/mL can bind Anti Human CD276 Antibody with the EC50 of 10.73 ng/mL. 2、Immobilized Recombinant Human CD276 Protein (rp143912) at 1 μg/mL can bind Anti Human CD276 Antibody with a linear range of 1 - 4 ng/mL (EC50:4.3 ng/mL).
38.9 kDa, under reducing conditions; 37.0 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
Reconstitute in sterile H2O to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Store at ﹣20~﹣80℃ for more than 1 year.
Images
Recombinant Human CD276 Protein (rp143912) - Protein Bioactivity Immobilized Recombinant Human CD276 Protein (rp143912) at 0.5 μg/mL can bind Anti Human CD276 Antibody with the EC50 of 10.73 ng/mL.
Recombinant Human CD276 Protein (rp143912) - Protein Bioactivity Immobilized Recombinant Human CD276 Protein (rp143912) at 1 μg/mL can bind Anti Human CD276 Antibody with a linear range of 1 - 4 ng/mL (EC₅₀:4.3 ng/mL).
Recombinant Human CD276 Protein (rp143912) - SDS-PAGE 3 μg/lane of Recombinant Human CD276 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.9 kDa under reducing conditions and 37.0 kDa under non-reducing conditions.
Recombinant Human CD276 Protein (rp143912) - ELISA Immobilized Recombinant Human CD276 Protein ( rp143912) at 1.0 μg/mL can bind Mirzotamab (anti-CD276) (Ab182952) with the EC₅₀ of 28.84 ng/mL.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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