Recombinant Src Antibody - Primary antibody, specific to Src, Rabbit IgG

Cat. No.: Ab129134

GRADE & PURITY Recombinant, ExactAb™, KD Validation, Validated, See COA

Application

WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human Src Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant Src Antibody - Primary antibody, specific to Src, Rabbit IgG

Synonyms

ASV1 | THC6 | c-SRC | p60-Src | SRC | ASV | EC 2.7.10 | EC 2.7.10.2 | pp60c-src | Rous sarcoma | RSVgp4 | tyrosine kinase pp60c-src | tyrosine-protein kinase SRC-1 | v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog | v-src sarcoma (Schmidt-

Specifications & Purity

Recombinant, ExactAb™, KD Validation, Validated, See COA

Biochemical and Physiological Mechanisms

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this prot

Host species

Rabbit

Specificity

Src

Immunogen

A synthetic peptide derived from human Src (AA 250-536)

Positive Control

WB: HeLa, Jurkat, Hep G2, MCF7, HEK293, A431, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, KD Validation, Recombinant, Validated

Isotype

Rabbit IgG

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Product Line

Biological Products

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant Src Antibody (Ab129134)

Recombinant Src Antibody (Ab129134) - Western Blot
All lanes: Recombinant Src Antibody (Ab129134) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 59 kDa
Exposure time: 100.0 s

Application

Application

Dilution info

WB

1/1000 - 1/2000

Associated Targets(Human)

SRC Tclin Proto-oncogene tyrosine-protein kinase Src (0 Activities)

Discover Target: SRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SRC Tclin Tyrosine-protein kinase SRC (10310 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin VEGF-receptor 2 and tyrosine-protein kinase SRC (25 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin Calmodulin/Proto-oncogene tyrosine-protein kinase Src (2 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin VHL/SRC (1 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SRC Tclin Src/Lck kinase (1 Activities)

Discover Target: SRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Application Protocols

Tested applications, recommended dilutions, incubation times/temperatures, antigen-retrieval conditions, and positive control samples are not specified for this item; refer to the CoA/Spec Sheet for authoritative application guidance.

General reminder: Application performance can vary with sample type, fixation, and buffer composition. Validate conditions empirically in your laboratory and include appropriate positive/negative controls. Do not substitute this general note for item-specific protocols.

Biological Roles

Target context (literature, general):

• Src (c-Src) is a prototypical non-receptor tyrosine kinase of the Src family kinases (SFKs) with modular SH3–SH2–kinase domain architecture and myristoylation at the N-terminus for membrane association. It regulates signaling pathways controlling adhesion, cytoskeletal dynamics, migration, proliferation, and survival.
• Regulation involves intramolecular interactions: phosphorylation of C-terminal Tyr527 (human) promotes a closed, autoinhibited conformation via SH2 engagement, whereas autophosphorylation at Tyr416 within the activation loop enhances catalytic activity.
• Upstream inputs include integrins, receptor tyrosine kinases (EGFR, PDGFR), GPCRs, and focal adhesion components (FAK). Downstream substrates encompass focal adhesion proteins (paxillin, FAK itself), adaptor molecules, and components of MAPK and PI3K pathways.
• Cellular/organismal roles: Src contributes to focal adhesion turnover, endocytosis, and cell cycle control. Dysregulated Src activity has been widely studied in the context of aberrant signaling in model systems; however, this product is for research use only.

Implications for antibody use (general):

• Antibodies to Src are used to detect total Src, specific phosphorylation states (e.g., pTyr416, pTyr527) or domain-specific epitopes in immunoblotting, immunoprecipitation, immunofluorescence, and kinase-proximity studies. For this specific item, the epitope/phospho-specificity and application validations are not specified; consult the CoA/Spec Sheet for tested features.

Buffer Applications

Item-specific buffer composition and recommended diluents are not provided and should be confirmed on the CoA/Spec Sheet. General guidance for antibody handling in common applications:

• Working buffers (general, literature best practice):
  • PBS or TBS at neutral pH (7.2–7.6) for routine dilutions; add 0.01–0.05% non-ionic surfactant (e.g., Tween-20) for immunoblotting or ELISA to reduce nonspecific binding, if compatible with the antibody.
  • Include carrier proteins (e.g., 0.1–1% BSA) where adsorption to plastics is a concern; confirm compatibility with downstream assays.
  • For cell-based assays, use azide-free buffers to avoid inhibition of peroxidase-based detection and to maintain cell viability; sterile-filter all buffers.
• Sample preparation considerations:
  • For immunoprecipitation, maintain isotonic buffers and consider mild detergents (NP-40/Triton X-100) at low percentages to preserve protein–protein interactions; adjust salt to modulate stringency.
  • For kinase signaling studies, include phosphatase and protease inhibitors during lysis to preserve Src phosphorylation states.

Note: The presence/absence of preservatives, stabilizers, and exact pH for this item are not specified. Always cross-check the product datasheet to align buffers with the supplied formulation.

Green Alternatives

Antibodies are inherently aqueous biomolecules and are commonly supplied in water-based buffers, aligning with green chemistry principles by avoiding volatile organic solvents during use.

• General considerations for greener practice with antibodies:
  • Prefer phosphate- or HEPES-buffered saline over organic cosolvents; limit glycerol to only what is necessary for freeze–thaw stabilization if cold-chain reliability is high.
  • If azide-free handling is feasible (e.g., for live-cell applications), use sterile, filtered buffers and low-protein-binding plastics to maintain stability without biocides, recognizing the tradeoff of reduced antimicrobial protection.
  • Minimize single-use plastics by planning aliquots efficiently; adopt recyclable cold packs for short shipments and validated reusable shippers where permitted.
  • Avoid harsh detergents unless required for the application; choose mild non-ionic surfactants at minimal effective concentrations to reduce environmental load.

Tradeoffs (general): Removing preservatives reduces environmental and user exposure but increases contamination risk; lower glycerol content improves viscosity/handling and reduces carbon footprint but may reduce freeze–thaw resilience. Evaluate based on your lab’s storage and sterility controls. Item-specific formulation details for this product are not specified; consult the CoA/Spec Sheet.

Pharmaceutical Uses

This product is designated For research use only and is not intended for human or veterinary therapeutic or diagnostic use.

Within pharmaceutical and bioprocess laboratories (non-clinical contexts), antibodies like this are commonly employed as:

• Analytical reagents for characterization of signaling pathways in cell lines and process-development models.
• QC/assay reagents in potency or identity assays for research materials, and as controls in immunoblotting or ELISA supporting discovery workflows.
• Tools for target validation and mechanism-of-action studies in preclinical research models.

No pharmacopeial status, GMP grade, or clinical applicability is claimed in the Product Data. Item-specific details such as endotoxin level, bioburden, residual host-cell protein/DNA, and compliance with compendial chapters are not specified for this item; refer to the CoA/Spec Sheet if your workflow requires defined quality attributes for regulated environments.

Physical Properties

Item-specific physico-chemical properties are not provided for this recombinant antibody and should be obtained from the CoA/Spec Sheet. Antibodies are aqueous biomacromolecules; standard solvent-style properties (bp, mp, logP) are not applicable.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. (Literature: full-length IgG often ~150 kDa; fragments vary.)
• Solubility: Antibodies are typically soluble in buffered aqueous solutions (e.g., PBS, Tris-based) with stabilizers; specific buffer for this item is not provided.
• Isoelectric point (pI): Not specified for this item; refer to CoA/Spec Sheet. (Literature values for IgG pI commonly ~6–9, clone-dependent.)
• Extinction coefficient (A280): Not specified for this item; refer to CoA/Spec Sheet. (General: IgG mass extinction coefficient ~1.4 (mg/mL)−1 cm−1, literature.)
• Stability considerations (general): Temperature-sensitive; avoid elevated temperatures and repeated freeze–thaw cycles to minimize aggregation and activity loss.

Because this product is protein-based, typical small-molecule properties such as boiling point, melting point, density, and refractive index are not applicable and are therefore not listed.

Quality & Grades

• Grade/Purity (as provided): Recombinant, ExactAb™, KD Validation, Validated.

Interpretation and implications (general, non-item-specific explanations):

• Recombinant antibody: Produced from defined cloned sequences in a controlled expression system (e.g., mammalian or other host). Advantages typically include lot-to-lot sequence identity and reduced variability compared with hybridoma-derived monoclonals or polyclonals.
• ExactAb™: Trademarked designation by the supplier/manufacturer; typically denotes a precisely defined recombinant antibody format. For this item, the specific criteria underlying the mark are not detailed—refer to the CoA/Spec Sheet for exact specifications (sequence, isotype, host system, purity method).
• KD Validation: Indicates binding affinity characterization has been performed (e.g., KD determination by SPR/BLI or equivalent). The numerical KD value, assay platform, and conditions are not specified here; consult the datasheet for binding parameters and acceptance criteria.
• Validated: The product has undergone internal validation; however, validated applications, species reactivity, and performance claims are not enumerated in the Product Data and must be verified on the CoA.

What is not specified for this item (obtain from CoA/Spec Sheet): isotype/subclass, clonality, concentration, purity metrics (e.g., % monomer by SEC), endotoxin level, host/expression system, formulation (buffer, preservatives), and tested applications. These attributes directly influence assay performance and should be reviewed for QC-sensitive workflows.

Reaction & Applications

This section is not typically applicable to antibodies. Recombinant Src Antibody is used as a binding reagent in immunoassays rather than as a reactant/solvent in chemical synthesis. For practical usage contexts, refer to Application Protocols (when available on the CoA/Spec Sheet) and Biological Roles for target context.

Reaction Conditions

Not applicable in the chemical reaction sense. Antibody performance depends on assay conditions rather than stoichiometric reaction parameters. Conditions such as incubation time, temperature, and buffer composition are application-specific and must be obtained from the product’s CoA/Spec Sheet or validated empirically in your lab for techniques like WB, IP, IF, IHC, or ELISA (if applicable).

Safety & Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to the SDS for authoritative safety information.
• General laboratory safety (good practice for protein antibodies):
  • Wear appropriate PPE: lab coat, gloves (nitrile), and safety glasses.
  • Handle in a clean environment to avoid microbial contamination; use sterile tips/tubes when asepsis is required.
  • Avoid aerosol generation and splashes; work in a biosafety cabinet if handling biological samples in conjunction.
• Storage incompatibilities and stability (general):
  • Avoid repeated freeze–thaw cycles, which can denature or aggregate antibodies—aliquot upon first thaw.
  • Do not mix with strong oxidants, proteases, or metal-catalyzed oxidative systems; avoid extreme pH (<5 or >9) unless the antibody is engineered/validated for such conditions.
  • Presence of sodium azide (if any) is not specified for this item; if present, avoid contact with metals (risk of explosive metal azides) and dispose of azide-containing waste properly.
• First-aid (general guidance; defer to SDS):
  • Skin/eye contact: Rinse with water. Remove contaminated clothing. Seek medical attention if irritation persists.
  • Ingestion/inhalation: Rinse mouth, move to fresh air, and obtain medical advice if symptoms occur.
• Transport: Shipped in ice chest with ice pads (per Product Data) to maintain cold chain integrity.

Always consult the product-specific SDS and institutional biosafety protocols before use.

Solvent Selection

Not typically applicable. As a recombinant antibody supplied for biochemical assays, it is intended for use in aqueous biological buffers rather than organic solvents. For practical considerations on buffers and diluents, see Buffer Applications and Storage & Reconstitution tabs.

Storage & Reconstitution

• Storage conditions (item-specific): Store at -20°C. Avoid repeated freezing and thawing. Shipped in an ice chest with ice pads to maintain the cold chain.
• Aliquoting: To comply with the “avoid repeated freezing and thawing” instruction, divide into single-use or limited-use aliquots upon initial receipt/thaw. Use low-protein-binding tubes.
• Freeze–thaw management: Thaw gently on ice. Do not heat to accelerate thawing. Mix by gentle inversion; avoid vortexing to limit aggregation.
• Light and contamination: Protect from microbial contamination by using sterile technique. Protect from prolonged exposure to strong light if conjugated; no conjugation is specified for this item.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If supplied lyophilized (not specified), reconstitute only with the recommended buffer to the stated concentration.
• Short-term handling: Keep on ice during use. For bench-top work exceeding 1–2 hours, consider chilled blocks to maintain temperature; exact stability window is not specified.
• Long-term storage: No data beyond “Store at -20°C” is provided. Many antibodies are stable at -80°C for extended storage (general practice), but item-specific guidance should be followed from the datasheet.

Always refer to the product-specific datasheet for formulation details (buffer, stabilizers, preservatives) and any additional storage instructions.

Structure & Identity

Recombinant Src Antibody is a proteinaceous affinity reagent directed against the Src proto-oncogene product (c-Src), a non-receptor tyrosine kinase. As a biologic macromolecule, it does not have small-molecule identifiers (SMILES/InChI) or a defined elemental molecular formula in the sense used for organic compounds.

• Item-specific identifiers
  • Product name: Recombinant Src Antibody (ExactAb™, KD Validation)
  • Category: Antibody (research use)
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES: Not applicable to recombinant antibodies; not specified for this item.
• Structural features (general, literature)
  • Typical antibody architecture: two heavy chains and two light chains linked by disulfides, forming an H2L2 immunoglobulin with variable domains (VH/VL) providing antigen recognition and constant domains (CH/CL) conferring isotype-specific properties.
  • If IgG (common for research antibodies), the quaternary structure is a Y-shaped homodimer of heavy–light chain pairs; glycosylation at Fc is typical and host/expression-system dependent.
  • Approximate mass for a full-length IgG is commonly ~150 kDa (literature; item-specific mass/isotype not provided).
• Antigen identity (general)
  • Target: c-Src (SRC gene), a cytoplasmic tyrosine kinase with SH3–SH2–kinase domain organization; regulatory Tyr527 (human numbering) and activation loop Tyr416 are canonical features.

Note: Epitope, clone, isotype, host species, and sequence are not specified for this item; consult the CoA/Spec Sheet for definitive identity attributes.

Synthetic Utility

Not applicable. As a recombinant antibody, this material is a biological affinity reagent rather than a synthetic building block or catalyst. It is not typically used to mediate or undergo organic reactions in a synthetic chemistry context. For its laboratory value, see Biological Roles, Buffer Applications, and Application Protocols (when available).

Target Specificity

• Antigen/Target: Src (c-Src) non-receptor tyrosine kinase (general target identity inferred from product name). Item-specific epitope, phospho-specificity, and cross-reactivity profile are not provided.
• Clone ID, isotype/subclass, host/expression system, and species reactivity: Not specified for this item; refer to CoA/Spec Sheet.

Notes (general):

• Src-family homology can lead to cross-reactivity among SFKs (e.g., Yes, Fyn) depending on epitope selection; actual specificity for this item is not stated and should be confirmed on the datasheet or by in-house testing.
• If phospho-specific, the antibody would recognize defined tyrosine phosphorylation sites (e.g., Tyr416 or Tyr527); no such claim is made in the provided Product Data.

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Src Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.