Recombinant UBE4B Antibody - Primary antibody, specific to UBE4B, Rabbit IgG

Cat. No.: Ab133294
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.3 mg/mL
Application
IF/ICC, WB
Host species
Rabbit
Reactive species
Human, Mouse, Rat
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab133294-10μl
1
$99.90
50μl
Ab133294-50μl
1
$259.90
100μl
Ab133294-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab133294-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human UBE4B Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
ICC/IF: 1/50-1/200
Protein Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Post-translational
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.

Specifications

Product Name
Recombinant UBE4B Antibody - Primary antibody, specific to UBE4B, Rabbit IgG
Synonyms
4930551I19Rik antibody | 4933406G05Rik antibody | AU014668 antibody | D4Bwg0973e antibody | E4 antibody | HDNB1 antibody | Homozygously deleted in neuroblastoma 1 antibody | KIAA0684 antibody | mKIAA0684 antibody | RP23 173D8.1 antibody | Ube4b antibody |
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species
Rabbit
Specificity
UBE4B
Immunogen
A synthetic peptide derived from human UBE4B (AA 28-40).
Positive Control
WB: HeLa, A549, U-2 OS, K562, MCF7, NIH/3T3 and C6 cell lysates. ICC/IF: MCF7 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.3 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant UBE4B Antibody (Ab133294) - Western Blot
All lanes: Recombinant UBE4B Antibody (Ab133294) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 146 kDa
Observed band size: 138 kDa
Exposure time: 5.4 s

Recombinant UBE4B Antibody (Ab133294) - IF/ICC
Immunocytochemistry analysis of UBE4B (green) in MCF7 using Recombinant UBE4B Antibody (Ab133294) and DAPI (blue).

Application
ApplicationDilution info
IF/ICC

1/50-1/200

WB

1/500-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
UBE4B Tbio Ubiquitin conjugation factor E4 B (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0809276Certificate of AnalysisMay 21, 2026 Ab133294
ZJ24F0809277Certificate of AnalysisMay 21, 2026 Ab133294
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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