GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
ExactAb™, Validated, Recombinant, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Vinculin Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Specifications
Product Name
Recombinant Vinculin Antibody - Primary antibody, specific to Vinculin
ExactAb™, Validated, Recombinant, High Performance, See COA
Biochemical and Physiological Mechanisms
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and l
Host species
Rabbit
Specificity
Vinculin
Immunogen
A synthetic peptide derived from human Vinculin (AA 1000-1078).
Positive Control
WB: MCF7, HeLa and C6 cell lysates. IF/ICC: A673 cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Vinculin Antibody (Ab134233) - Western Blot All lanes: Recombinant Vinculin Antibody (Ab134233) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 124 kDa Observed band size: 120 kDa Exposure time: 58.8 s
Recombinant Vinculin Antibody (Ab134233) - IF/ICC Immunofluorescent analysis of A673 cells, using Recombinant Vinculin Antibody (Ab134233).
Application
Application
Dilution info
IF/ICC
1/50-1/200
WB
1/1000 - 1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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