AIF Mouse mAb - Primary antibody, specific to AIFM1, Mouse IgG2a

Cat. No.: Ab087780

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

8H1-B10-A12

Conjugation

Unconjugated

Descripción general

Mouse anti Human AIF Antibody, Monoclonal(8H1-B10-A12), could be used for WB, IF, ICC and so on .
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Specifications

Product Name

AIF Mouse mAb - Primary antibody, specific to AIFM1, Mouse IgG2a

Sinónimos

AIF | AIFapoptosis-inducing factor 1, mitochondrial | AIFM1 | apoptosis-inducing factor, mitochondrion-associated, 1 | COXPD6 | MGC111425 | PDCD8 | PDCD8striatal apoptosis-inducing factor | programmed cell death 8 (apoptosis-inducing factor) | Programmed

Especificaciones y pureza

ExactAb™, Validated, 1.0 mg/mL

Especies hospedadoras

Mouse

Especificidad

AIFM1

Inmunógeno

Recombinant protein of human AIF (AA 1-170)

Control positivo

WB: HeLa, Ramos, HepG2, MCF-7, Jurkat lysates. ICC/IF: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: AIF Mouse mAb (Ab087780)

AIF Mouse mAb (Ab087780) - Western Blot
All lanes: AIF Mouse mAb (Ab087780) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 66 kDa
Exposure time: 24.0 s

IF/ICC: AIF Mouse mAb (Ab087780)

AIF Mouse mAb (Ab087780) - IF/ICC
Immunocytochemistry analysis of AIF in HeLa using AIF Mouse mAb (Ab087780).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

Objetivos asociados (humanos)

AIFM1 Tbio Apoptosis-inducing factor 1, mitochondrial (0 Activities)

Discover Target: AIFM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción