GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human AIMP3/p18 Antibody, Monoclonal (E94S639), could be used for WB, IF/ICC and so on.
Specifications
Product Name
AIMP3/p18 Mouse mAb - Primary antibody, specific to EEF1E1, Mouse IgG2b
Sinónimos
AIMP 3 | AIMP3 | Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 | Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 | ARS interacting multifunctional protein 3 | Cyclin dependent kinase 6 inhibitor | Elongati
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Mecanismos bioquímicos y fisiológicos
Positive modulator of ATM response to DNA damage.
Especies hospedadoras
Mouse
Especificidad
EEF1E1
Inmunógeno
Recombinant fusion protein of human EEF1E1.
Control positivo
WB: Mouse testis, Rat brain, Hep G2, A549, A431, Jurkat and K562 lysates. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG2b
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
AIMP3/p18 Mouse mAb (Ab087803) - Western Blot All lanes: AIMP3/p18 Mouse mAb (Ab087803) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 12.0 s
AIMP3/p18 Mouse mAb (Ab087803) - Western Blot All lanes: AIMP3/p18 Mouse mAb (Ab087803) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 25.5 s
AIMP3/p18 Mouse mAb (Ab087803) - IF/ICC Immunofluorescent analysis of EEF1E1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AIMP3/p18 Mouse mAb (Ab087803) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Aplicación
Aplicación
Dilution info
ICC/IF
1/10-1/50
WB
1/500-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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