GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human AKAP 95 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
AKAP 95 Antibody - Primary antibody, specific to AKAP8, Rabbit IgG
Sinónimos
A kinase (PRKA) anchor protein 8 antibody | A kinase anchor protein 8 antibody | A kinase anchor protein 95 antibody | A kinase anchor protein 95 kDa antibody | A kinase anchor protein 95kDa antibody | A-kinase anchor protein 8 antibody | A-kinase anchor
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Mecanismos bioquímicos y fisiológicos
Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).
Especies hospedadoras
Rabbit
Especificidad
AKAP8
Inmunógeno
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKAP 95.
Control positivo
WB: Hep G2, Jurkat and HEK293 cell lysates. IHC: Human heart tissue. IF/ICC: Jurkat cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
Método de purificación
Immunogen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
AKAP 95 Antibody (Ab087835) - Western Blot All lanes: AKAP 95 Antibody (Ab087835) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 76 kDa Observed band size: 91 kDa Exposure time: 60.0 s
AKAP 95 Antibody (Ab087835) - Western Blot All lanes: AKAP 95 Antibody (Ab087835) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 76 kDa Observed band size: 91 kDa Exposure time: 60.0 s
AKAP 95 Antibody (Ab087835) - Western Blot Immunohistochemical analysis of AKAP8 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with AKAP 95 Antibody (Ab087835) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
AKAP 95 Antibody (Ab087835) - IF/ICC Immunofluorescent analysis of AKAP8 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AKAP 95 Antibody (Ab087835) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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