Goat Anti-Rabbit IgG H&L (FITC) - Secondary antibody

Cat. No.: Ab170148

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:498nm, Em:517nm, Pre-adsorbed, 1.0 mg/mL

Application

IF/ICC

Host species

Goat

Reactive species

Rabbit

Conjugation

FITC

Descripción general

Application
IF: 1:1000

Specifications

Product Name

Goat Anti-Rabbit IgG H&L (FITC) - Secondary antibody

Sinónimos

Goat Anti-Rabbit IgG H&L Antibody (FITC) | Anti-Rabbit IgG H&L Antibody (FITC) | Rabbit IgG H&L Antibody (FITC) | FITC-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (FITC) | Anti-Rabbit IgG Antibody (FITC) | Rabbit IgG Antibody (FI

Especificaciones y pureza

ExactAb™, Azide Free, Validated, Ex:498nm, Em:517nm, Pre-adsorbed, 1.0 mg/mL

Especies hospedadoras

Goat

Inmunógeno

Rabbit IgG

Conjugación

FITC

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grado

Azide Free, Em:517nm, Ex:498nm, ExactAb™, Pre-adsorbed, Validated

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Imágenes

IF: Goat Anti-Rabbit IgG H&L (FITC) (Ab170148)

Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) - IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1:1000;