Goat Anti-Rabbit IgG H&L (Biotin) - Secondary antibody, Goat IgG

Cat. No.: Ab176442
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 1.0 mg/mL
Application
ELISA, WB
Host species
Goat
Reactive species
Rabbit
Isotype
Goat IgG
Conjugation
Biotin
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
100μg
Ab176442-100μg
≥10
31,90US$
1mg
Ab176442-1mg
2
239,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing Ships Wet ice Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application
WB: 1:10,000-1:200,000

ELISA: 1:10,000-1:200,000

Specifications

Product Name
Goat Anti-Rabbit IgG H&L (Biotin) - Secondary antibody, Goat IgG
Sinónimos
Goat Anti-Rabbit IgG H&L Antibody (Biotin) | Anti-Rabbit IgG H&L Antibody (Biotin) | Rabbit IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (Biotin) | Anti-Rabbit IgG Antibody (Biotin) | Rabbit IgG
Especificaciones y pureza
ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL
Especies hospedadoras
Goat
Inmunógeno
Rabbit IgG
Conjugación
Biotin
Grado
Azide Free, ExactAb™, High Performance, Validated
Isotipo
Goat IgG
Método de purificación
Antigen affinity purified
Concentración
1.0 mg/mL
Nota
This product can replace Ab138234
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Enviado en
Wet ice
Estabilidad y almacenamiento
Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Imágenes

Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) at 1/40000 dilution
SA-HRP:1:20000

Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 7.0 s

Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) - ELISA
Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab176442) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.

Aplicación
AplicaciónDilution info
WB

1/10000-1/200000

ELISA

1:10000-1:200000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ23F1101827Certificate of AnalysisMar 17, 2026 Ab176442
ZJ23F1101828Certificate of AnalysisMar 17, 2026 Ab176442
ZJ24R1201653Certificate of AnalysisDec 26, 2024 Ab176442
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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