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Goat Anti-Rabbit IgG H&L (HRP) - Secondary antibody, Goat IgG

Cat. No.: Ab176443
COA
Goat Anti-Rabbit IgG H&L (HRP) - by Aladdin Scientific
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.
Application
ELISA, WB
Host species
Goat
Reactive species
Rabbit
Isotype
Goat IgG
Conjugation
HRP
★
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
100μg
Ab176443-100μg
≥10
35,90US$
1mg
Ab176443-1mg
5
189,90US$
Enter a quantity for the sizes you want to add.
Bulk / Custom
♡ Lista de deseos ⇅ Añadir para comparar ⎙ Print page
SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Descripción general Specifications Almacenamiento y envío Imágenes Aplicación Documentation Advanced Data Certificados (CoA, COO, BSE/TSE y tabla de análisis) Preguntas frecuentes y artículos Calculadoras de soluciones Reseñas
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application
WB: 1:10,000-1:200,000
ELISA: 1:10,000-1:100,000

Specifications

Product Name
Goat Anti-Rabbit IgG H&L (HRP) - Secondary antibody, Goat IgG
Sinónimos
Goat Anti-Rabbit IgG H&L Antibody (HRP) | Anti-Rabbit IgG H&L Antibody (HRP) | Rabbit IgG H&L Antibody (HRP) | Peroxidase-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (HRP) | Anti-Rabbit IgG Antibody (HRP) | Rabbit IgG Antibody (H
Especificaciones y pureza
ExactAb™, High Performance, Validated, Azide Free
Especies hospedadoras
Goat
Inmunógeno
Rabbit IgG
Control positivo
1.0 mg/mL
Conjugación
HRP
Grado
Azide Free, ExactAb™, High Performance, Validated
Isotipo
Goat IgG
Método de purificación
Antigen affinity purified
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Imágenes

Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/40000 dilution

Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 2.0 s

Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) - ELISA
Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) was used as the secondary antibody.

Aplicación
AplicaciónDilution info
WB

1/10000-1/200000

ELISA

1:10000-1:100000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

7 results found

Lot NumberCertificate TypeFechaArticulo
ZJ23F1101823Certificate of AnalysisMar 17, 2026 Ab176443
ZJ23F1101825Certificate of AnalysisMar 17, 2026 Ab176443
ZJ24F0809959Certificate of AnalysisMar 17, 2026 Ab176443
ZJ24F0809960Certificate of AnalysisMar 17, 2026 Ab176443
ZJ26F0332361Certificate of AnalysisMar 03, 2026 Ab176443
ZJ26F0332360Certificate of AnalysisMar 03, 2026 Ab176443
ZJ24R0500573Certificate of AnalysisMay 19, 2024 Ab176443
Preguntas frecuentes y artículos
In-cell ELISA protocol
Categories: Protocols
Tags:
Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies
Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol
Categories: Protocols
Tags:
Cytokines Growth factors ELISPOT Secreted Proteins HRP Conjugated Secondary Antibodies
Automated Immunohistochemical Staining Protocol for Anti-PD-L1
Categories: Protocols
Tags:
PD-L1 IHC Monoclonal antibodies Automated Immunohistochemistry Isotype Control Antibodies
General Protocol for Western Blotting
Categories: Protocols
Tags:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP
BN-PAGE Protocol
Categories: Protocols
Tags:
Western blotting Natural polypropylene gel electrophoresis Non-denaturating gel electrophoresis BN-PAGE Coomasil brilliant blue Protein complex Horseradish peroxidase Alkaline phosphatase
Histone Western Blot
Categories: Protocols
Tags:
Monoclonal antibodies Western blot Recombinant Antibodies Immunoblotting HRP Horseradish peroxidase Histone
Protocol of Western blot for Phosphorylated Proteins
Categories: Protocols
Tags:
Western blot ECL Phosphorylated proteins Phosphorylated Antibodies Immunoblot HRP-conjugated Antibodies
Guidelines of Secondary Antibody for Western Blotting
Categories: Protocols
Tags:
Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Multiplex Immunohistochemistry Protocol
Categories: Protocols
Tags:
Immunoglobulin Immunohistochemical staining Immunofluorescence TSA HRP labeled secondary antibody Tyrosine amplification signal Multiple immunohistochemistry
Western Blot Protocol
Categories: Protocols
Tags:
WB SDS-PAGE Western blot Cell lysis Protein extraction electrophoresis buffer protease inhibitor phosphatase inhibitor ECL hypersensitive luminescent solution gel preparation chemiluminescent detection
Immunohistochemistry Protocol
Categories: Protocols
Tags:
IHC Immunohistochemistry Antibody detection Immunohistochemistry Protocol Antigen retrieval solution Wash buffer Fixative Paraffin Section Experiment Frozen Section Experiment Tissue Staining Technique
Horseradish Peroxidase (HRP) — Structural Features, Enzymatic Properties and Application Overview
Categories: Technical articles
Tags:
Peroxidase
Tyramide Signal Amplification in In Situ Fluorescence Detection
Categories: Technical articles
Tags:
HRP TSA tyramide signal amplification in situ fluorescence detection multiplex immunofluorescence FFPE FISH
Protein Carbonyl Detection
Categories: Technical articles
Tags:
Mass spectrometry Immunoblotting Oxidative Stress sample pretreatment DNPH Protein Carbonyls Protein Oxidation
Practical Guide to TSA Tyramide Signal Amplification: How to Select Reagents, Optimize the Workflow, and Reduce Background When Detection Signals Are Weak for Low-Abundance Targets
Categories: Technical articles
Tags:
ICC IHC Immunohistochemistry Horseradish peroxidase In situ hybridization TSA tyramide signal amplification Fluorescence In Situ Hybridization
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →
Bulk & Custom Packaging Need larger quantities or custom specifications? Contact our team for a tailored quote.

View related series

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