GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.Pre-adsorbed?Pre-adsorbed — cross-reactive antibodies removed by adsorption for higher specificity. Use in multiplex/IHC to cut background and cross-reaction.1.0 mg/mL
ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL Azide Free,ExactAb™,High Performance,Pre-adsorbed,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Biotin conjugated anti-rabbit secondary antibody is commonly used for immunohistochemistry, Western blotting, and ELISA. Application WB: 1:10000-1:200000 ELISA: 1:10000-1:100000 IF: 1:1000
ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL
Especies hospedadoras
Goat
Inmunógeno
Rabbit IgG
Conjugación
Biotin
Grado
Azide Free, ExactAb™, High Performance, Pre-adsorbed, Validated
Concentración
1.0 mg/mL
Nota
This product can replace Ab138234
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Enviado en
Wet ice
Estabilidad y almacenamiento
Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.
Imágenes
Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) - IF IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) - ELISA Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) was tested by direct ELISA. The plate was coated with 100 ng of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Aplicación
Aplicación
Dilution info
WB
1/10000-1/200000
IF/ICC
1:1000
ELISA
1:10000-1:100000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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