ATP5H Antibody - Primary antibody, specific to ATP5H, Rabbit IgG

Cat. No.: Ab090134

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human ATP5H Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/100-1/300
Protein Function:
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Specifications

Product Name

ATP5H Antibody - Primary antibody, specific to ATP5H, Rabbit IgG

Sinónimos

ATP synthase D chain mitochondrial antibody | ATP synthase H+ transporting mitochondrial F1F0 subunitantibody | ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody | ATP synthase subunit d antibody | ATP synthase subunit d, mitochondrial an

Especificaciones y pureza

ExactAb™, Validated, 1.0 mg/mL

Especies hospedadoras

Rabbit

Especificidad

ATP5H

Inmunógeno

A synthetic peptide derived from human ATP5H (AA 111-160).

Control positivo

WB: Hep G2, Jurkat and HeLa cell lysates. IHC: Human Squamous cell carcinoma of lung tissue.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: ATP5H Antibody (Ab090134)

ATP5H Antibody (Ab090134) - Western Blot
All lanes: ATP5H Antibody (Ab090134) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 22 kDa
Exposure time: 14.0 s

IHC: ATP5H Antibody (Ab090134)

ATP5H Antibody (Ab090134) - IHC
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung.
1. ATP5H Antibody (Ab090134) was diluted at 1/200 (4 ℃, overnight).
2. Tris-EDTA, pH 9.0 was used for antigen retrieval.
3. Secondary antibody was diluted at 1/200 (room temperature, 45 min).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/100-1/300

Objetivos asociados (humanos)

ATP5PD Tbio ATP synthase subunit d, mitochondrial (0 Activities)

Discover Target: ATP5PD

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)