CALIDAD & PUREZARecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Recombinant, ExactAb™, KD Validation, Validated, See COA ExactAb™,KD Validation,Recombinant,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Rabbit anti Human E2F1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Especificaciones
Product Name
Recombinant E2F1 Antibody - Primary antibody, specific to E2F1, Rabbit IgG
Recombinant, ExactAb™, KD Validation, Validated, See COA
Mecanismos bioquímicos y fisiológicos
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF
Especies hospedadoras
Rabbit
Especificidad
E2F1
Inmunógeno
A synthetic peptide derived from human E2F1 (AA 365-400)
Control positivo
WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, KD Validation, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Product Line
Productos biológicos
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant E2F1 Antibody (Ab100782) - Western Blot All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 66, 74 kDa Exposure time: 3.8 s
Recombinant E2F1 Antibody (Ab100782) - Western Blot All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).
Recombinant E2F1 Antibody (Ab100782) - IHC Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).
Aplicación
Aplicación
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentación
📋 Hoja de datos de seguridad (SDS)
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
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Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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