CD14 Mouse mAb (Biotin) - Primary antibody, specific to CD14

Cat. No.: Ab210089

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

UCHM-1

Conjugation

Biotin

Descripción general

Mouse anti Human CD14 Antibody, Monoclonal (UCHM-1), could be used for Flow, WB, IHC, IF, ICC, IP and so on.

Specifications

Product Name

CD14 Mouse mAb (Biotin) - Primary antibody, specific to CD14

Sinónimos

CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec

Especies hospedadoras

Mouse

Especificidad

CD14

Inmunógeno

Human Thymocytes followed by peripheral blood mononuclear cells

Control positivo

Flow: Human peripheral blood monocytes

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD14 Mouse mAb (Biotin) (Ab210089)

CD14 Mouse mAb (Biotin) (Ab210089) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD14 Mouse mAb (Biotin) (Ab210089) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: CD14 Mouse mAb (Biotin) (Ab210089)

CD14 Mouse mAb (Biotin) (Ab210089) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD14 Mouse mAb (Biotin) (Ab210089) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (Biotin) (Ab180306). Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)

Discover Target: CD14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción