Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L

Cat. No.: Ab210600

CALIDAD & PUREZA Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC

Host species

Mouse

Reactive species

Virus

Isotype

Mouse IgG2b

Clone Number

EF11/6E10

Conjugation

Unconjugated

Descripción general

Mouse IgG2b, Isotype Control Antibody (EF11/6E10), could be used for Flow Cytometry analysis, IF/ICC, ELISA and so on.  

Especificaciones

Product Name

Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L

Sinónimos

Mouse IgG2b, κ Isotype Control | Purified Mouse IgG2b, kappa Isotype Control | Mouse IgG2b Isotype Control Antibody | Mouse IgG2b Isotype Control mAb | Immunoglobulin G2B Fc | Mouse IgG2B Isotype Control | Isotype control mAb for mouse IgG2b | IgG2b (Mous

Especificaciones y pureza

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two he

Especies hospedadoras

Mouse

Especificidad

E8L

Inmunógeno

Recombinant protein corresponding to MPXV E8L

Control positivo

Flow Cytometry: Peripheral blood mononuclear cells; Mouse splenocytes IF/ICC: HeLa cells

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Flow Cytometry: Mouse IgG2b-kappa, anti-E8L (Ab210600)

Mouse IgG2b-kappa, anti-E8L (Ab210600) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood mononuclear cells labelling CD45RA (Red) with Recombinant CD45RA Antibody (Ab212152) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, compared to concentration matched Mouse IgG2b-kappa, anti-E8L (Ab210600) (Blue) conjugated with biotin. Detected with SA-PE (rp156150).

Flow Cytometry: Mouse IgG2b-kappa, anti-E8L (Ab210600)

Mouse IgG2b-kappa, anti-E8L (Ab210600) - Flow Cytometry
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling Mouse IgG2b (Left panel) with Mouse IgG2b-kappa, anti-E8L (Ab210600) conjugated with biotin at 10.0 μg/mL for 1 hour at 4°C, compared with unlabelled control (Right panel). Detected with SA-PE (rp156150).

ICC/IF: Mouse IgG2b-kappa, anti-E8L (Ab210600)

Mouse IgG2b-kappa, anti-E8L (Ab210600) - ICC/IF
IF analysis of HeLa cells using beta Tubulin Mouse mAb (Ab179922) (Green) compared to concentration matched Mouse IgG2b-kappa, anti-E8L (Ab210600). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used at a dilution of 1/1000. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

Aplicación

Dilution info

Flow Cytometry

Use at the same concentration as the detection antibody.

IF/ICC

Use at the same concentration as the detection antibody.

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Mouse IgG2b-kappa, anti-E8L); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.