CD47 Mouse mAb (Biotin) - Primary antibody, specific to CD47, Mouse IgG1

Cat. No.: Ab185151

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

CC2C6

Conjugation

Biotin

Descripción general

Application:
Flow: ≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD47 Mouse mAb (Biotin) - Primary antibody, specific to CD47, Mouse IgG1

Sinónimos

antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein |

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Mouse

Especificidad

CD47

Inmunógeno

CCRF-CEM T-cell line.

Control positivo

Flow: MCF-7

Conjugación

Biotin

Grado

ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD47 Mouse mAb (Biotin) (Ab185151)

CD47 Mouse mAb (Biotin) (Ab185151) - Flow Cytometry
Flow cytometry analysis of CD47 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD47 Mouse mAb (Biotin) (Ab185151) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. ;

Objetivos asociados (humanos)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción