GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human CDK5RAP1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Specifications
Product Name
CDK5RAP1 Antibody - Primary antibody, specific to CDK5RAP1, Rabbit IgG
Sinónimos
2310066P17Rik antibody | C20orf34 antibody | C42 antibody | CDK5 activator binding protein C42 antibody | CDK5 activator binding protein C42 like antibody | CDK5 regulatory subunit associated protein 1 antibody | CDK5RAP1.3 antibody | CDK5RAP1.4 antibody
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Mecanismos bioquímicos y fisiológicos
Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i6A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe,
Especies hospedadoras
Rabbit
Especificidad
CDK5RAP1
Inmunógeno
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK5RAP1.
Control positivo
WB: Mouse lung lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant CDK5RAP1 Antibody (Ab095771) - Western Blot All lanes: Recombinant CDK5RAP1 Antibody (Ab095771) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 68 kDa Observed band size: 61 kDa Exposure time: 27.0 s
Recombinant CDK5RAP1 Antibody (Ab095771) - IHC Immunohistochemical analysis of CDK5RAP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant CDK5RAP1 Antibody (Ab095771) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CDK5RAP1 Antibody (Ab095771) - IF/ICC IF analysis of CDK5RAP1 Antibody (Ab095771) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDK5RAP1 Antibody (Ab095771) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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