Cell adhesion molecule 4 Antibody - Primary antibody, specific to CADM4, Rabbit IgG

Cat. No.: Ab095999

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human Cell adhesion molecule 4 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50 - 1/100
IF/IC :1/50 - 1/200
Protein Function
Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.

Specifications

Product Name

Cell adhesion molecule 4 Antibody - Primary antibody, specific to CADM4, Rabbit IgG

Sinónimos

TSLL2 antibody | TSLC1-like protein 2 antibody | TSLC1 like protein 2 antibody | CADM4_HUMAN | cadm4 | Cell adhesion molecule 4 | IgSF4C | Immunoglobulin superfamily member 4C | TSLC1 like 2 antibody | synCAM4 antibody | Nectin-like protein 4 antibody | N

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Rabbit

Especificidad

CADM4

Inmunógeno

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CADM4.

Control positivo

WB: Mouse brain, Rat brain, U-2 OS and C6 lysates. IHC: Human brain tissue. IF/ICC: A549 cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Cell adhesion molecule 4 Antibody (Ab095999)

Cell adhesion molecule 4 Antibody (Ab095999) - Western Blot
All lanes: Cell adhesion molecule 4 Antibody (Ab095999) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 56, 72 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 0.3 s

IF/ICC: Cell adhesion molecule 4 Antibody (Ab095999)

Cell adhesion molecule 4 Antibody (Ab095999) - IF/ICC
Immunofluorescent analysis of CADM4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Cell adhesion molecule 4 Antibody (Ab095999) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IHC: Cell adhesion molecule 4 Antibody (Ab095999)

Cell adhesion molecule 4 Antibody (Ab095999) - IHC
Immunohistochemical analysis of CADM4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Cell adhesion molecule 4 Antibody (Ab095999) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Aplicación

• Aplicación: Dilution info
• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

• WB:

  1/500-1/1000 ;

Objetivos asociados (humanos)

CADM4 Tbio Cell adhesion molecule 4 (0 Activities)

Discover Target: CADM4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)