COL25A1 Antibody - Primary antibody, specific to COL25A1, Rabbit IgG

Cat. No.: Ab191534

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human COL25A1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.

Specifications

Product Name

COL25A1 Antibody - Primary antibody, specific to COL25A1, Rabbit IgG

Sinónimos

Alzheimer disease amyloid associated protein antibodyAlzheimer disease amyloid-associated protein antibody | AMY antibody | CLAC antibody | CLAC P antibody | CLAC precursor antibody | CLAC-P antibody | Col25a1 antibody | COL25A1 protein antibody | Collage

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin. Post-translational: Undergoes proteolytic cleavage by furin protease to yield

Especies hospedadoras

Rabbit

Especificidad

COL25A1

Inmunógeno

A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 25 alpha 1.

Control positivo

WB: HEK293 and HeLa cell lysates. IHC: Human brain tissue. IF/ICC: Hep G2 cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

COLWestern Blot: COL25A1 Antibody (Ab191534) 25A1 Antibody

COL25A1 Antibody (Ab191534) - Western Blot
All lanes: COL25A1 Antibody (Ab191534) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 99 kDa
Exposure time: 4.9 s

IHC: COL25A1 Antibody (Ab191534)

COL25A1 Antibody (Ab191534) - IHC
Immunohistochemical analysis of Collagen 25 alpha 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with COL25A1 Antibody (Ab191534) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: COL25A1 Antibody (Ab191534)

COL25A1 Antibody (Ab191534) - IF/ICC
Immunofluorescent analysis of Collagen 25 alpha 1 staining in Hep G2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the COL25A1 Antibody (Ab191534) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/50 - 1/200;

• IHC:

  1/50-1/200;

Objetivos asociados (humanos)

COL25A1 Tbio Collagen alpha-1(XXV) chain (0 Activities)

Discover Target: COL25A1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)