CstF-64 Antibody - Primary antibody, specific to CSTF2, Rabbit IgG

Cat. No.: Ab098002

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cow, Human, Mouse, Rat, Zebrafish

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human CstF-64 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on.
Application：
WB: 1/500-1/1000
IHC:1/50 - 1/100
IF/ICC: 1/50-1/200
Protein Function：
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.

Specifications

Product Name

CstF-64 Antibody - Primary antibody, specific to CSTF2, Rabbit IgG

Sinónimos

CSTF64 | CSTF2 | CstF-64 | XLID113 | betaCstF 64 variant 2 antibody | CF-1 64 kDa subunit antibody | CF1 64 kDa subunit antibody | Cleavage stimulation factor 2 antibody | Cleavage stimulation factor 64 kDa subunit antibody | Cleavage stimulation factor s

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Rabbit

Especificidad

CSTF2

Inmunógeno

A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CstF-64.

Control positivo

WB: K562 cell lysate. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: CstF-64 Antibody (Ab098002)

CstF-64 Antibody (Ab098002) - Western Blot
All lanes: CstF-64 Antibody (Ab098002) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 67 kDa
Exposure time: 11.3 s

IHC: CstF-64 Antibody (Ab098002)

CstF-64 Antibody (Ab098002) - IHC
Immunohistochemical analysis of CstF-64 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with CstF-64 Antibody (Ab098002) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: CstF-64 Antibody (Ab098002)

CstF-64 Antibody (Ab098002) – IF/ICC
Immunofluorescent analysis of CstF-64 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CstF-64 Antibody (Ab098002) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Aplicación

• Aplicación: Dilution info
• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

• WB:

  1/500-1/1000 ;

Objetivos asociados (humanos)

CSTF2 Tbio Cleavage stimulation factor subunit 2 (0 Activities)

Discover Target: CSTF2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)