CXCR4/CD184 Mouse mAb - Primary antibody, specific to CXCR4/CD184, Mouse IgG2a

Cat. No.: Ab098344

GRADE & PURITY Carrier Free, Validated, ExactAb™, Low Endotoxin, Azide Free, PBS Only, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Blocking, Flow Cytometry, Functional Assay, IF/ICC, IHC, Neutralization, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

12G5

Conjugation

Unconjugated

Descripción general

Mouse anti Human CXCR4/CD184 Antibody, Monoclonal (12G5), could be used for Block, Flow, ICC, IF, IHC, Neutralization, WB and so on.
Application
Block: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Neutralization: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Protein Function
Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.

Specifications

Product Name

CXCR4/CD184 Mouse mAb - Primary antibody, specific to CXCR4/CD184, Mouse IgG2a

Sinónimos

WHIM | WHIMS1 | D2S201E | CD184 | chemokine (C-X-C motif) receptor 4 | chemokine (C-X-C motif), receptor 4 (fusin) | C-X-C chemokine receptor type 4 | CXCR4 | CXC-R4 | CXCR-4 | D2S201ESDF-1 receptor | FB22 | Fusin | HM89 | HM89NPYRL | HSY3RR | LAP3 | LAP-

Especificaciones y pureza

Carrier Free, ExactAb™, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Especies hospedadoras

Mouse

Especificidad

CXCR4/CD184

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, Low Endotoxin, PBS Only, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

Método de purificación

Protein A/G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -80°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Flow Cytometry: CXCR4/CD184 Mouse mAb (Ab098344)

CXCR4/CD184 Mouse mAb (Ab098344) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CXCR4/CD184 with CXCR4/CD184 Mouse mAb (Ab098344) conjugated with biotin (right panel) at 1.0 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CXCR4/CD184 Mouse mAb (Ab098344)

CXCR4/CD184 Mouse mAb (Ab098344) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CXCR4/CD184 (red) with CXCR4/CD184 Mouse mAb (Ab098344) conjugated with biotin at 1.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

SEC: CXCR4/CD184 Mouse mAb (Ab098344)

CXCR4/CD184 Mouse mAb (Ab098344) - SEC
The purity of CXCR4/CD184 Mouse mAb (Ab098344) is more than 95% verified by HPLC.

Aplicación

• Aplicación: Dilution info
• WB:

  Use at an assay dependent concentration.;

• Neutralization:

  Use at an assay dependent concentration.

• IHC:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• Blocking:

  Use at an assay dependent concentration.

Objetivos asociados (humanos)

CXCR4 Tclin C-X-C chemokine receptor type 4 (0 Activities)

Discover Target: CXCR4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción