DNAJA2 Mouse mAb - Primary antibody, specific to DNAJA2

Cat. No.: Ab100138

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Dog, Human, Monkey, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

OTI3A10

Conjugation

Unconjugated

Descripción general

Rabbit anti Human DNAJA2 Antibody, Monoclonal (OTI3A10), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

DNAJA2 Mouse mAb - Primary antibody, specific to DNAJA2

Sinónimos

DNAJA2 | DJ3 | CPR3 | DJA2 | DNAJ | DNJ3 | RDJ2 | HIRIP4 | PRO3015 | Cell cycle progression 3 protein antibody | Cell cycle progression restoration gene 3 protein antibody | CPR 3 antibody | CPR3 antibody | Dj3 antibody | DJA 2 antibody | DJA2 antibody |

Especificaciones y pureza

ExactAb™, Validated, See COA

Especies hospedadoras

Mouse

Especificidad

DNAJA2

Inmunógeno

Recombinant Full length protein of human DNAJA2.

Control positivo

WB: Hep G2, HT-29, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human breast, kidney, ovary, thyroid, endometrium, bladder, lymph node, lymphoma tissue. IF/ICC: COS7 cells transiently transfected by pCMV6-ENTRYDNAJA2. Flow Cytometry: HEK293T cel

Conjugación

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - Western Blot
All lanes: DNAJA2 Mouse mAb (Ab100138) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 100.0 s

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using DNAJA2 Mouse mAb (Ab100138). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IHC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using DNAJA2 Mouse mAb (Ab100138). (Heatinduced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab100138, Dilution 1/50)

IF/ICC: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - IF/ICC
DNAJA2 Mouse mAb (Ab100138) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2.

Flow Cytometry: DNAJA2 Mouse mAb (Ab100138)

DNAJA2 Mouse mAb (Ab100138) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by DNAJA2 Mouse mAb (Ab100138), and then analyzed by flow cytometry.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000 ;

• IF/ICC:

  1/100;

• Flow Cytometry:

  1/100;

• IHC:

  1/50-1/150 ;

Objetivos asociados (humanos)

DNAJA2 Tbio DnaJ homolog subfamily A member 2 (0 Activities)

Discover Target: DNAJA2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (DNAJA2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.