GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human DNAJC3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.
Specifications
Product Name
DNAJC3 Antibody - Primary antibody, specific to DNAJC3, Rabbit IgG
Sinónimos
DnaJ (Hsp40) homolog subfamily C member 3 antibody | DnaJ homolog subfamily C member 3 antibody | DNAJC3 antibody | DNJC3_HUMAN antibody | double-stranded RNA-activated protein kinase inhibitor antibody | Endoplasmic reticulum DnaJ protein 6 antibody | ER
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Mecanismos bioquímicos y fisiológicos
Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein
Especies hospedadoras
Rabbit
Especificidad
DNAJC3
Inmunógeno
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DnaJC3.
Control positivo
WB: COLO 205 and Raji cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: SGC7901 cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
DNAJC3 Antibody (Ab100175) - Western Blot All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 57 kDa Exposure time: 3.7 s
DNAJC3 Antibody (Ab100175) - Western Blot All lanes: DNAJC3 Antibody (Ab100175) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 57 kDa Exposure time: 3.7 s
DNAJC3 Antibody (Ab100175) - IHC Immunohistochemical analysis of DNAJC3 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DNAJC3 Antibody (Ab100175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
DNAJC3 Antibody (Ab100175) - IF/ICC Immunofluorescent analysis of DNAJC3 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DNAJC3 Antibody (Ab100175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Aplicación
Aplicación
Dilution info
WB
1/500-1/2000
IHC
1/50 - 1/100
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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