E8L Mouse mAb - Primary antibody, specific to E8L

Cat. No.: Ab179942

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Detection Antibody, ELISA

Host species

Mouse

Reactive species

Virus

Isotype

Mouse IgG2b

Clone Number

GH02/2H12

Conjugation

Unconjugated

Descripción general

Mouse anti MPXV E8L Antibody, clone GH02/2H12, could be used as a detection antibody in ELISA application.

Especificaciones

Product Name

E8L Mouse mAb - Primary antibody, specific to E8L

Sinónimos

E8L Antibody | Monkeypox Virus E8L Antibody | MPXV E8L Antibody | Anti-Monkeypox virus/MPXV E8L Antibody | Monoclonal Anti-Monkeypox-E8L Antibody | anti-Virus E8L | Monkeypox Virus Cell Surface-Binding Protein (MPXV E8L) Antibody

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

Especies hospedadoras

Mouse

Especificidad

E8L

Inmunógeno

Recombinant protein corresponding to MPXV E8L

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Pares emparejados

[Ab156143] E8L Mouse mAb, Capture Antibody

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Sandwich ELISA - E8L Antibody Pair (Ab156143 & Ab179942)

Sandwich ELISA - E8L standard curve
Sandwich ELISA detection of Recombinant MPXV E8L Protein (rp155937) using E8L Mouse mAb (Ab156143) as capture antibody at concentration of 1.0 µg/mL. Biotinylated E8L Mouse mAb (Ab179942) as detection antibody at concentration of 1.0 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.008 ng/mL - 125.0 ng/mL

Indirect ELISA - E8L Mouse mAb (Ab179942)

Indirect ELISA - E8L standard curve
Immobilized Recombinant MPXV E8L Protein (rp155937) at 1.0 µg/mL overnight at 4℃. Coated protein probed with E8L Mouse mAb (Ab179942). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 5.267 ng/mL

Aplicación

Aplicación

Dilution info

ELISA

0.5-2000 ng/mL

Detection Antibody

1.0 μg/mL

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (E8L Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.