GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human EGFR Antibody, Monoclonal(C4), could be used for WB, IHC and so on.
Specifications
Product Name
EGFR Mouse mAb - Primary antibody, specific to EGFR, IgG2b
Carrier Free, ExactAb™, Validated, High Performance, See COA
Mecanismos bioquímicos y fisiológicos
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
Especies hospedadoras
Mouse
Especificidad
EGFR
Inmunógeno
Recombinant EGFR expressed in E.coli (AA 888-1210)
Control positivo
WB: A431, HeLa, MCF7, A549, NIH/3T3 and HUVEC cell lysates. IHC: Human kidney, placent, lung cancer, stomach, adrenal gland, liver cancer tissues. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
IgG2b
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A + Protein G purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
EGFR Mouse mAb (Ab101163) - Western Blot All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 175 kDa Observed band size: 169 & 69 kDa Exposure time: 10.4 s
EGFR Mouse mAb (Ab101163) - Western Blot All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 175 kDa Observed band size: 169 & 69 kDa Exposure time: 2.3 s
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Lung cancer Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Stomach Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Adrenal gland Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Liver cancer Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IF/ICC IF analysis of EGFR Mouse mAb (Ab101163) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with EGFR Mouse mAb (Ab101163) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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