Factor B Antibody - Primary antibody, specific to Factor B, Rabbit IgG

Cat. No.: Ab102641

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Application

IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human Factor B Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
Protein Function:
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

Especificaciones

Product Name

Factor B Antibody - Primary antibody, specific to Factor B, Rabbit IgG

Sinónimos

AHUS4 antibody | B factor properdin antibody | BF antibody | BFD antibody | C3 proaccelerator antibody | C3 proactivator antibody | C3/C5 convertase antibody | CFAB antibody | CFAB_HUMAN antibody | CFB antibody | Complement Factor B antibody | Complement

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Especies hospedadoras

Rabbit

Especificidad

Factor B

Inmunógeno

Recombinant CFB expressed in E.coli (AA 270-388).

Control positivo

WB: Human serum lysate. IHC: Human spleen, liver cancer; Mouse kidney, liver tissues.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Antigen affinity purified + Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Factor B Antibody (Ab102641)

Factor B Antibody (Ab102641) - Western Blot
All lanes: Factor B Antibody (Ab102641) at 1/1000 dilution
Samples: Lysates at 20 µL per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 92 kDa
Exposure time: 10.0 s 

IHC: Factor B Antibody (Ab102641)

Factor B Antibody (Ab102641) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 20 µg/mL Factor B Antibody (Ab102641)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Factor B Antibody (Ab102641)

Factor B Antibody (Ab102641) - IHC
DAB staining on IHC
Sample: Mouse Liver Tissue
Primary Ab: 20 µg/mL Factor B Antibody (Ab102641)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Factor B Antibody (Ab102641)

Factor B Antibody (Ab102641) - IHC
DAB staining on IHC
Sample: Mouse Kidney Tissue
Primary Ab: 20 µg/mL Factor B Antibody (Ab102641)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Factor B Antibody (Ab102641)

Factor B Antibody (Ab102641) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 20 µg/mL Factor B Antibody (Ab102641)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Aplicación

Aplicación

Dilution info

IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Objetivos asociados (humanos)

CFB Tchem Complement factor B (0 Activities)

Discover Target: CFB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Factor B Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.