Feline CD16 Mouse mAb - Primary antibody, specific to Feline CD16, Mouse IgG1

Cat. No.: Ab185361

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Cat

Isotype

Mouse IgG1

Clone Number

AB02/2E5

Conjugation

Unconjugated

Descripción general

Flow: 1/3,000 - 1/200,000

Especificaciones

Product Name

Feline CD16 Mouse mAb - Primary antibody, specific to Feline CD16, Mouse IgG1

Sinónimos

CD 16 antibody | CD 16a antibody | CD16 antibody | CD16a antibody | CD16a antigen antibody | CD16B antibody | CD16b antigen antibody | Fc fragment of IgG antibody | Fc fragment of IgG low affinity IIIa receptor (CD16) antibody | Fc fragment of IgG low aff

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Especies hospedadoras

Mouse

Especificidad

Feline CD16

Inmunógeno

Recombinant protein corresponding to Feline CD16

Control positivo

Flow: Feline Peripheral Mononuclear Cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

lambda

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Flow Cytometry: Feline CD16 Mouse mAb (Ab185361)

Feline CD16 Mouse mAb (Ab185361) - Flow Cytometry
Flow Cytometry analysis of cat peripheral blood lymphocytes labeling CD16 with Feline CD16 Mouse mAb (Ab185361) (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) (left panel) using the Goat Anti-Mouse IgG H&L (PE) (Ab179006) as the secondary antibody.​

Flow Cytometry: Feline CD16 Mouse mAb (Ab185361)

Feline CD16 Mouse mAb (Ab185361) - Flow Cytometry
Flow Cytometry analysis of cat peripheral blood lymphocytes labeling CD16 with Feline CD16 Mouse mAb (Ab185361) (red) at 0.1 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabeled control, cells without incubation with primary antibody.​

Flow Cytometry: Feline CD16 Mouse mAb (Ab185361)

Feline CD16 Mouse mAb (Ab185361) - Flow Cytometry
Flow Cytometry analysis of cat peripheral blood lymphocytes labelling CD16 with Feline CD16 Mouse mAb (Ab185361) conjugated with biotin (right panel) at 0.6 μg/mL for 1 hour at 4°C, compared with unlabeled control (left panel), detected with SA-PE (rp156150). ​

Flow Cytometry: Feline CD16 Mouse mAb (Ab185361)

Feline CD16 Mouse mAb (Ab185361) - Flow Cytometry
Flow Cytometry analysis of cat peripheral blood lymphocytes labelling CD16 with Feline CD16 Mouse mAb (Ab185361) (red) conjugated with biotin at 0.6 μg/mL for 1 hour at 4°C, detected with SA-PE (rp156150). Black - Unlabeled control, cells without incubation with primary antibody.​

Aplicación

Aplicación

Dilution info

Flow Cytometry

1/3,000 - 1/200,000

Objetivos asociados (humanos)

FCGR3A Tclin Low affinity immunoglobulin gamma Fc region receptor III-A (0 Activities)

Discover Target: FCGR3A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Feline CD16 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.