GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1, 4, 5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusiv
Especies hospedadoras
Rabbit
Especificidad
FFAR3
Inmunógeno
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human FFAR3/GPR41.
Control positivo
WB: Mouse kidney, Mouse liver, Rat liver, HeLa, Jurkat and RAW 264.7 lysates. IF/ICC: LOVO cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
FFAR3/GPR41 Antibody (Ab103246) - Western Blot All lanes: FFAR3/GPR41 Antibody (Ab103246) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 28.1 s
FFAR3/GPR41 Antibody (Ab103246) - Western Blot All lanes: FFAR3/GPR41 Antibody (Ab103246) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 30 s
FFAR3/GPR41 Antibody (Ab103246) - IF/ICC Immunofluorescent analysis of GPR41 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FFAR3/GPR41 Antibody (Ab103246) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Aplicación
Aplicación
Dilution info
IF/ICC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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