GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, validado, sin portador, 1,0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
El anticuerpo de conejo anti GAB1 humano, policlonal, se puede utilizar para WB, IHC, ICC, IF, etc. Aplicación: WB: 1/500-1/1000 IHC: 1/100-1/200 ICC/IF: 1/100-1/500 Función Proteica： Probablemente implicada en la señalización de los receptores de EGF e insulina.
Specifications
Product Name
Anticuerpo GAB1 - Primary antibody, specific to GAB1, Rabbit IgG
Sinónimos
Anticuerpo Gab 1 | Anticuerpo GAB1 | Anticuerpo GAB1_HUMAN | Anticuerpo GRB 2 associated binder 1 | Anticuerpo GRB2 associated binding protein 1 isoform a | Anticuerpo GRB2 associated binding protein 1 isoform b | GRB2-as
Especificaciones y pureza
ExactAb™, validado, sin portador, 1, 0 mg/mL
Especies hospedadoras
Rabbit
Especificidad
GAB1
Inmunógeno
A synthetic peptide derived from human GAB1
Control positivo
WB: HEK293, Hep G2 and K562 cell lysates. IHC: Human breast cancer tissue. IF/ICC: Hep G2 cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Líquido
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (12 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.
Imágenes
GAB1 Antibody (Ab104356) - Western Blot All lanes: GAB1 Antibody (Ab104356) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 77 kDa Observed band size: 107 kDa Exposure time: 120.0 s
GAB1 Antibody (Ab104356) - IHC Immunohistochemical analysis of GAB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GAB1 Antibody (Ab104356) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
GAB1 Antibody (Ab104356) - IF/ICC Immunofluorescent analysis of GAB1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GAB1 Antibody (Ab104356) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Aplicación
Aplicación
Dilution info
IHC
1/100 - 1/200
WB
1/500-1/1000
ICC/IF
1/100-1/500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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