GNMT Mouse mAb - Primary antibody, specific to GNMT, Mouse IgG2a

Cat. No.: Ab105966

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

Mouse

Reactive species

Human, Mouse

Isotype

Mouse IgG2a

Clone Number

A41S18

Conjugation

Unconjugated

Descripción general

Mouse anti Human GNMT Antibody, Monoclonal (A41S18), could be used for WB, Flow and so on.

Specifications

Product Name

GNMT Mouse mAb - Primary antibody, specific to GNMT, Mouse IgG2a

Sinónimos

EC 2.1.1.20 antibody | Epididymis secretory sperm binding protein Li 182mP antibody | Glycine N methyltransferase antibody | Glycine N-methyltransferase antibody | Gnmt antibody | GNMT_HUMAN antibody | HEL S 182mP antibody | OTTHUMP00000016412 antibody |

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet a

Especies hospedadoras

Mouse

Especificidad

GNMT

Inmunógeno

Recombinant protein of human GNMT.

Control positivo

WB: Mouse liver lysates. Flow: U-2 OS cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: GNMT Mouse mAb (Ab105966)

GNMT Mouse mAb (Ab105966) - Western Blot
All lanes: GNMT Mouse mAb (Ab105966) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 20.8 s

Flow Cytometry: GNMT Mouse mAb (Ab105966)

GNMT Mouse mAb (Ab105966) - Flow Cytometry
Flow cytometric analysis of U-2 OS cells using GNMT Mouse mAb (Ab105966). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/10-1/50;

Objetivos asociados (humanos)

GNMT Tchem Glycine N-methyltransferase (0 Activities)

Discover Target: GNMT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción